| Co-hydrolysis of lignocellulosic biomass for microbial lipid accumulation. | |
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MedLine Citation:
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PMID: 23124976 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The herbaceous perennial energy crops miscanthus, giant reed, and switchgrass, along with the annual crop residue corn stover, were evaluated for their bioconversion potential. A co-hydrolysis process, which applied dilute acid pretreatment, directly followed by enzymatic saccharification without detoxification and liquid-solid separation between these two steps was implemented to convert lignocellulose into monomeric sugars (glucose and xylose). A factorial experiment in a randomized block design was employed to optimize the co-hydrolysis process. Under the optimal reaction conditions, corn stover exhibited the greatest total sugar yield (glucose + xylose) at 0.545 g·g(-1) dry biomass at 83.3% of the theoretical yield, followed by switch grass (0.44 g·g(-1) dry biomass, 65.8% of theoretical yield), giant reed (0.355 g·g(-1) dry biomass, 64.7% of theoretical yield) and miscanthus (0.349 g·g(-1) dry biomass, 58.1% of theoretical yield). The influence of combined severity factor on the susceptibility of pretreated substrates to enzymatic hydrolysis was clearly discernible, showing that co-hydrolysis is a technically feasible approach to release sugars from lignocellulosic biomass. The oleaginous fungus Mortierella isabellina was selected and applied to the co-hydrolysate mediums to accumulate fungal lipids due to its capability of utilizing both C5 and C6 sugars. Fungal cultivations grown on the co-hydrolysates exhibited comparable cell mass and lipid production to the synthetic medium with pure glucose and xylose. These results elucidated that combining fungal fermentation and co-hydrolysis to accumulate lipids could have the potential to enhance the utilization efficiency of lignocellulosic biomass for advanced biofuels production. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc. |
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Authors:
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Zhenhua Ruan; Michael Zanotti; Yuan Zhong; Wei Liao; Chad Ducey; Yan Liu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-1 |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: - ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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