| Co-expression of renin-angiotensin system component genes in human atrial tissue. | |
| | |
MedLine Citation:
|
PMID: 7965269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: To detect co-expression of genes coding for components of the renin-angiotensin system and investigate the potential for variation in the level of angiotensin converting enzyme (ACE) gene expression in the right atrial appendage of patients undergoing heart surgery. DESIGN: The right atrial appendage was collected at the time of surgery from 30 randomly chosen patients and was rapidly frozen in liquid nitrogen prior to extraction of messenger (m)RNA. Surgical samples of heart valve (n = 6) and papillary muscle (n = 3) were also examined. METHODS: Aliquots of purified mRNA were reverse-transcribed for analysis of gene expression by a polymerase chain reaction amplification assay. Primers specific for angiotensinogen, renin, ACE, cardiac chymase, atrial natriuretic peptide, glyceraldehyde-3-phosphate dehydrogenase, adenosine deaminase and the transferrin receptor were used for a qualitative analysis of co-expression of these genes within the same sample. In a subgroup of eight patients, a quantitative comparison of the relative levels of ACE gene expression was performed using a competitive polymerase chain reaction. RESULTS: Angiotensinogen and ACE expression were detected in all atrial, valve and ventricular samples examined, at levels similar to those of 'housekeeping' genes such as the transferrin receptor. Atrial renin and chymase expression were more difficult to detect, being demonstrable in only 70 and 63% of the samples, respectively. Higher levels of chymase were detected in ventricular samples than in atrial tissues. A quantitative analysis of ACE expression in eight atrial samples provided evidence of interindividual variation in the relative level of atrial ACE expression. CONCLUSIONS: The essential components of the renin-angiotensin system are co-expressed at a low level in the right atrial appendage and are detectable in other regions of the human heart. Renin and chymase genes are expressed at a lower level than the angiotensinogen and ACE genes and exhibit regional differences in expression. Interindividual variation in the relative level of ACE expression can be detected by a competitive polymerase chain reaction. |
| | |
Authors:
|
K Morgan; J Wharton; J C Webb; B E Keogh; P L Smith; K M Taylor; C M Oakley; J M Polak; J G Cleland |
Related Documents
:
|
22544609 - Isolation and analysis of genes mainly expressed in adult mouse heart using subtractive... 8745219 - Characterization of cardiac gene cis-regulatory elements in the early stages of chicken... 19471099 - Transcriptional control of pacemaker channel genes hcn2 and hcn4 by sp1 and implication... 10570949 - Strain- and age-dependent loss of sarcoglycan complex in cardiomyopathic hamster hearts... 9510529 - Oxygen sensing and the transcriptional regulation of oxygen-responsive genes in yeast. 11223989 - Direct intra-cardiomuscular transfer of beta2-adrenergic receptor gene augments cardiac... 11342969 - Herpes simplex virus mediated nerve growth factor expression in bladder and afferent ne... 11927369 - Expression of c-fos gene activation during rough and tumble play in juvenile rats. 15120069 - The drosophila carbonyl reductase sniffer prevents oxidative stress-induced neurodegene... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of hypertension. Supplement : official journal of the International Society of Hypertension Volume: 12 ISSN: 0952-1178 ISO Abbreviation: J Hypertens Suppl Publication Date: 1994 Jul |
Date Detail:
|
Created Date: 1994-12-29 Completed Date: 1994-12-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8501422 Medline TA: J Hypertens Suppl Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: S11-9 Citation Subset: IM |
Affiliation:
|
Department of Clinical Cardiology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiotensinogen
/
genetics Atrial Natriuretic Factor / genetics Base Sequence Chymases DNA, Complementary / genetics Gene Expression* Glyceraldehyde-3-Phosphate Dehydrogenases / genetics Heart / physiology* Heart Atria Humans Molecular Sequence Data Oligonucleotide Probes / genetics Peptidyl-Dipeptidase A / genetics Polymerase Chain Reaction Renin-Angiotensin System / genetics* Serine Endopeptidases / genetics Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Complementary; 0/Oligonucleotide Probes; 11002-13-4/Angiotensinogen; 85637-73-6/Atrial Natriuretic Factor; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.39/Chymases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The advantages of angiotensin II antagonism.
Next Document: Acute and long-term effects of angiotensin converting enzyme inhibition on larger arteries and cardi...