| Co-expression of pendrin, vacuolar H+-ATPase alpha4-subunit and carbonic anhydrase II in epithelial cells of the murine endolymphatic sac. | |
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MedLine Citation:
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PMID: 15385584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The endolymph in the endolymphatic sac (ES) is acidic (pH 6.6-7). Maintaining this acidic lumen is believed to be important for the normal function of the ES. The acid-base regulation mechanisms of the ES are unknown. Here we investigated the expression patterns of acid-base regulators, including vacuolar (v)H+-ATPase (proton pump), carbonic anhydrase (CA) II, and pendrin in the murine ES epithelium by immunohistochemistry (IHC) and compared their expression patterns by double immunostaining. We found that pendrin and vH+-ATPase were co-localized in the apical membrane of a specific type of ES epithelial cell. Pendrin- and vH+-ATPase-positive cells also expressed cytoplasmic CA II. Co-expression of pendrin, vH+-ATPase, and CA II in the same subgroup of ES cells suggests that this specific type of ES cell is responsible for the acid-base balance processes in the ES and pendrin, vH+-ATPase, and CA II are involved in these processes. |
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Authors:
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Hongwei Dou; Jie Xu; Zhaohui Wang; Annabel N Smith; Manoocher Soleimani; Fiona E Karet; John H Greinwald; Daniel Choo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 52 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-23 Completed Date: 2004-11-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 1377-84 Citation Subset: IM |
Copyright Information:
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Copyright The Histochemical Society, Inc. |
Affiliation:
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Center for Hearing and Deafness Research, Department of Pediatric Otolaryngology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbonic Anhydrase II / biosynthesis* Carrier Proteins / biosynthesis* Endolymphatic Sac / cytology, enzymology* Epithelial Cells / enzymology* Fluorescent Antibody Technique Immunohistochemistry Membrane Transport Proteins* Mice Protein Subunits / biosynthesis Vacuolar Proton-Translocating ATPases / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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DC00193/DC/NIDCD NIH HHS; DK 54430/DK/NIDDK NIH HHS; DK 62829/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Membrane Transport Proteins; 0/Protein Subunits; 0/SLC26A4 protein, human; EC 3.6.1.-/Vacuolar Proton-Translocating ATPases; EC 4.2.1.-/Carbonic Anhydrase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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