Document Detail


Co-expression of d-glucose isomerase and d-psicose 3-epimerase: Development of an efficient one-step production of d-psicose.
MedLine Citation:
PMID:  25152409     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
d-Psicose has been attracting attention in recent years because of its alimentary activities and is used as an ingredient in a range of foods and dietary supplements. To develop a one-step enzymatic process of d-psicose production, thermoactive d-glucose isomerase and the d-psicose 3-epimerase obtained from Bacillus sp. and Ruminococcus sp., respectively, were successfully co-expressed in Escherichia coli BL21 strain. The substrate of one-step enzymatic process was d-glucose. The co-expression system exhibited maximum activity at 65°C and pH 7.0. Mg(2+) could enhance the output of d-psicose by 2.32 fold to 1.6g/L from 10g/L of d-glucose. When using high-fructose corn syrup (HFCS) as substrate, 135g/L d-psicose was produced under optimum conditions. The mass ratio of d-glucose, d-fructose, and d-psicose was almost 3.0:2.7:1.0, when the reaction reached equilibrium after an 8h incubation time. This co-expression system approaching to produce d-psicose has potential application in food and beverage products, especially softdrinks.
Authors:
Yan Men; Yueming Zhu; Yan Zeng; Ken Izumori; Yuanxia Sun; Yanhe Ma
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Publication Detail:
Type:  Journal Article     Date:  2014-06-20
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  64-65     ISSN:  1879-0909     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-08-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 Elsevier Inc. All rights reserved.
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