Document Detail

Co-expression of the TGF-beta1 and TGF- beta2 isoforms in nasal polyps and in healthy mucosa.
MedLine Citation:
PMID:  18059253     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The formation of nasal polyps is connected with a chronic inflammatory process with the activation of different cytokines. TGF-ss induces fibrosis and acts as a chemoattractant and proliferation factor for fibroblasts. The aim of the study was to evaluate the expression profiles of the genes coding TGF-ss isoforms in nasal polyps with predominately eosinophilic and neutrophilic infiltration and in healthy mucosa and to assess their mutual correlation with the levels of gene transcription. MATERIAL/METHODS: The study group consisted of 24 patients with nasal polyposis. On the basis of the histopathological evaluation there were 16 eosinophilic and 8 neutrophilic polyps. The control group constituted 9 healthy patients. The expression profiles of the genes coding the TGF-ss isoforms were detected using real-time RT-QPCR. RESULTS:TGF-beta1 and TGF-beta2 mRNAs were revealed in 10 patients with eosinophilic polyps. TGF-beta1 transcriptional activity was accompanied by TGF-beta2 transcriptional activity in nasal polyps. TGF-beta2 gene expression in tissues without mRNA for TGF-beta1 was silenced. There was positive correlation between the expressions of the TGF-beta1 and TGF-beta2 isoforms in nasal polyps. TGF-beta1 mRNA was present at higher levels in all control samples than in eosinophilic polyps. An increased TGF-beta1 mRNA expression was accompanied by an increased TGF-beta2 mRNA expression in healthy mucosa. TGF-beta3 showed the most intensive transcriptional activity among the TGF-ss isoforms in both nasal polyps and control tissues. There was no correlation between TGF-beta3 and TGF-beta1 nor between TGF-beta3 and TGF-beta2 transcriptional activity in nasal polyps and normal tissue.
Beata Rostkowska-Nadolska; Małgorzata Kapral; Urszula Mazurek; Wojciech Gawron; Krzysztof Preś
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-27
Journal Detail:
Title:  Postȩpy higieny i medycyny doświadczalnej (Online)     Volume:  61     ISSN:  1732-2693     ISO Abbreviation:  Postepy Hig Med Dosw (Online)     Publication Date:  2007  
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206517     Medline TA:  Postepy Hig Med Dosw (Online)     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  702-7     Citation Subset:  IM    
Faculty of Medicine, Department of Otorhinolaryngology, Wrocław Medical University, Wrocław, Poland.
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MeSH Terms
Case-Control Studies
Inflammation / metabolism*
Middle Aged
Nasal Mucosa / metabolism*
Nasal Polyps / genetics*,  metabolism
Protein Isoforms
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
Transforming Growth Factor beta / biosynthesis*
Transforming Growth Factor beta1 / biosynthesis
Transforming Growth Factor beta2 / biosynthesis
Transforming Growth Factor beta3 / biosynthesis
Reg. No./Substance:
0/Protein Isoforms; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 0/Transforming Growth Factor beta2; 0/Transforming Growth Factor beta3

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