| Co-cultures of human coronary smooth muscle cells and dimethyl sulfoxide-differentiated HL60 cells upregulate ProMMP9 activity and promote mobility-modulation by reactive oxygen species. | |
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MedLine Citation:
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PMID: 18665441 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vascular cells and leukocytes, involved in the development of atherosclerosis, produce cytokines and/or reactive oxygen species (ROS) and matrix metalloproteinases (MMPs) implicated in cell mobility. We investigated by co-culture experiments the effects of human coronary smooth muscle cells (HCSMC) on MMPs characteristics and mobility of neutrophil-like dimethyl sulfoxide-differentiated HL60 cells (not equal HL60). The effects of superoxide dismutase (SOD) and catalase were also analyzed. All the studied MMP2 characteristics remained unchanged. HCSMC stimulated MMP9 protein level, activity and mobility of not equal HL60 cells and expressed and secreted a variety of cytokines implicated in atherosclerosis. SOD and catalase increased MMP9 expression, protein level and activity of not equal HL60, but migration of not equal HL60 cells was only decreased by catalase, demonstrating that ROS are more efficient in modulating MMP9 activity of not equal HL60 than their mobility. Finally, HCSMC being able to stimulate not equal HL60, their co-cultures may represent an in vitro approach to study cellular interactions occurring in vivo during atherosclerosis. |
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Authors:
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Yohann Bernard; Chantal Melchior; Eric Tschirhart; Jean-Luc Bueb |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Inflammation Volume: 31 ISSN: 0360-3997 ISO Abbreviation: Inflammation Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-02 Completed Date: 2008-12-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: United States |
Other Details:
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Languages: eng Pagination: 287-98 Citation Subset: IM |
Affiliation:
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Life Sciences Research Unit, Université du Luxembourg, 162a, Avenue de la Faïencerie, 1511 Luxembourg, Luxembourg. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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metabolism Atherosclerosis / metabolism* Cells, Cultured Coculture Techniques* Dimethyl Sulfoxide / pharmacology* Gene Expression Regulation, Enzymologic* HL-60 Cells Humans Inflammation Matrix Metalloproteinase 9 / metabolism* Models, Biological Myocardium / metabolism* Myocytes, Smooth Muscle / cytology* Oxidative Stress RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/RNA, Messenger; 67-68-5/Dimethyl Sulfoxide; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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