Document Detail


Co-culture of carp (Cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of T-cell lineages.
MedLine Citation:
PMID:  19375173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To characterise fish haematopoietic stem/progenitor cells, it is necessary to develop a culture system that supports proliferation and differentiation of these cells. In the present study, we established cell lines from various tissues of carp (Cyprinus carpio) and ginbuna (Carassius auratus langsdorfii). By using these cell lines, we developed a culture system in which carp haematopoietic cells proliferated and were successively passaged. Cell lines from carp thymus (KoT), carp fin (KoF1) and ginbuna thymus (GTS6 and GTS9) were newly established. In addition to these cell lines, ginbuna fin (CFS) cell lines were also used as feeder layers. Kidney haematopoietic cells co-cultured with these feeder layers proliferated rapidly and were passaged over 20 times for more than 60 days. To characterise the proliferating cells, expression of marker genes for blood cell development were analysed. In the primary culture, marker genes for myeloid/erythroid progenitors (gata1), haematopoietic stem cells (gata2), neutrophils (mpx/mpo), B-cells (IgH) and T-cells (lck, TCRbeta and gata3) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). Expression of most of the genes disappeared after the third passage, only T-cell marker genes were highly expressed after passages. These results indicate that multiple blood cells developed in the primary culture and then T-cell lineages dominantly proliferated after several passages.
Authors:
Fumihiko Katakura; Fumio Takizawa; Miyuki Yoshida; Takuya Yamaguchi; Kyosuke Araki; Mitsuru Tomana; Miki Nakao; Tadaaki Moritomo; Teruyuki Nakanishi
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Publication Detail:
Type:  Journal Article     Date:  2009-03-20
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  131     ISSN:  1873-2534     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-18     Completed Date:  2009-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  127-36     Citation Subset:  IM    
Affiliation:
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carps / physiology*
Cell Line
Cell Lineage*
Coculture Techniques
Gene Expression
Goldfish
Hematopoietic Stem Cells / physiology*
Kidney / cytology*
Lymphocyte Activation*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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