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Co-culture of the 55-6 B cell hybridoma with the EL-4 thymoma cell. Effect on cell growth and monoclonal antibody production.
MedLine Citation:
PMID:  23765215     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The cell growth and monoclonal antibody production of the 55-6 hybridoma cell co-cultured with the murine thymoma cell line EL-4 at different initial 55-6:EL-4 ratios were investigated. Both populations were seeded in co-culture without previous stimulation and therefore with low constitutive CD40 and CD40 ligand (CD154) expression levels, and in the absence of exogenous co-stimuli. Viable cell density and growth rate data seem to suggest a competition for nutrients, which is detrimental for both cells in terms of biomass production and also of growth rate for 55-6. Final concentrations of antibody and specific antibody production rates were affected by the initial 55-6:EL-4 ratio. The 4:1 ratio yielded the highest IgG2a concentration, whereas the highest specific antibody production rate was obtained at the 2:1 ratio. Changes mainly in CD154 and also in CD40 expression in co-cultures could suggest cross-talk between both populations. In conclusion, different types of interactions are probably present in this co-culture system: competition for nutrients, cognate interaction and/or autocrine or paracrine interactions that influence the proliferation of both cells and the hybridoma antibody secretion. We are hereby presenting a pre-scale-up process that could speed up the optimization of large-scale monoclonal antibodies production in bioreactors by emulating the in vivo cell-cell interaction between B and T cells without previous stimulation or the addition of co-stimulatory molecules.
Authors:
Alicia Martín-López; Lourdes Acosta-López; Francisco García-Camacho; Antonio Contreras-Gómez; Emilio Molina-Grima
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Publication Detail:
Type:  Journal Article     Date:  2013-06-14
Journal Detail:
Title:  Cytotechnology     Volume:  65     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-24     Completed Date:  2013-07-24     Revised Date:  2013-08-02    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  655-62     Citation Subset:  -    
Affiliation:
Department of Chemical Engineering, University of Almería, Almería, Spain.
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