Document Detail


Clustering, migration, and neurite formation of neural precursor cells in the adult rat hippocampus.
MedLine Citation:
PMID:  17348003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult neurogenesis occurs in the subgranular zone and innermost part of the dentate granule cell layer. To examine how neural precursor cells proliferate, migrate, and extend their neurites, we performed BrdU- and improved retrovirus-green fluorescence protein (GFP)-labeling analyses. Soon after labeling the majority of BrdU+ cells and GFP+ cells expressed Ki67, a cell cycle marker, and formed clusters together with PSA+ neuroblasts. Most of the Ki67+ proliferating cells expressed Hu, an immature and mature neuronal marker, and the subpopulation expressed both Hu+ and GFAP+. In the clusters, Ki67+ and PSA+ cells strongly expressed beta-catenin and N-cadherin, but PSA+ cells outside the clusters did not. Therefore, it was mainly Hu+ neuronal precursor cells that proliferated within clusters in which the cluster cells are closely associated via cell adhesion molecules, such as N-cadherin/beta-cateninIn and PSA. The newly generated cells appeared to stay in the clusters for a few days and then disperse around the clusters. The findings of this in vivo analysis and in vitro time-lapse imaging of early postnatal hippocampal slices support the notion that most postmitotic neuroblasts migrate tangentially from clusters, extending tangentially oriented processes, one of which often retains close contact with the clusters, and finally extend radial processes, or prospective apical dendrites. These results suggest that the clustering cells and tangentially migrating cells have a systematic cellular arrangement and intercellular interaction.
Authors:
Tatsunori Seki; Takashi Namba; Hideki Mochizuki; Masafumi Onodera
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  502     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  275-90     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Juntendo University School of Medicine, Tokyo, Japan. tseki@med.juntendo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Animals, Newborn
Bromodeoxyuridine / metabolism
Cell Aggregation / physiology
Cell Count / methods
Cell Movement / physiology*
Hippocampus / cytology*
Male
Models, Biological
Nerve Tissue Proteins / metabolism
Neurites / physiology*
Neurons / cytology*,  physiology*
Organogenesis
Rats
Rats, Wistar
Retroviridae / physiology
Stem Cells / physiology*
Time Factors
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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