Document Detail

Clusterin protects against oxidative stress in vitro through aggregative and nonaggregative properties.
MedLine Citation:
PMID:  9607196     Owner:  NLM     Status:  MEDLINE    
Perturbations of cell interactions, an early event in acute renal injury, have important pathophysiologic consequences. We hypothesized that promotion of cell interactions protects cells from injury. To test this hypothesis, a single cell suspension of LLC-PK1 cells (porcine proximal tubular cell line) treated with albumin (control) was compared to cells aggregated with fibrinogen or purified human clusterin (aggregation graded 0 to 4). Following aggregation, the cells were injured with 1.5 mM hydrogen peroxide (H2O2) for three hours. Cell aggregation induced by clusterin but not fibrinogen protected against oxidant injury by H2O2. Complete abrogation of cytotoxicity occurred at a clusterin concentration of 2.5 micrograms/ml, which resulted in an aggregation score of 1. In the absence of aggregation, clusterin at concentrations of 20 and 50 micrograms/ml, but not lower doses, partially protected against injury induced by H2O2. Cell aggregation induced by both clusterin and fibrinogen partially protected against endogenously generated oxidant stress induced by incubating LLC-PK1 cells with aminotriazole and 1-chloro-2,4-dinitrobenzene (CDNB). In conclusion, clusterin protects against models of oxidant stress in vitro, whether generated by exogenously administered hydrogen peroxide, or from endogenously produced peroxide, and such protective effects can accrue from aggregative and nonaggregative properties of clusterin.
G B Schwochau; K A Nath; M E Rosenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Kidney international     Volume:  53     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-08-03     Completed Date:  1998-08-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1647-53     Citation Subset:  IM    
Department of Medicine, University of Minnesota, Minneapolis, USA.
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MeSH Terms
Amitrole / pharmacology
Cell Aggregation / drug effects
Cell Communication / drug effects
Cell Death / drug effects
Dinitrochlorobenzene / pharmacology
Dose-Response Relationship, Drug
Fibrinogen / pharmacology
Glycoproteins / pharmacology*
Irritants / pharmacology
Kidney Tubules, Proximal / cytology,  drug effects*
LLC-PK1 Cells
Molecular Chaperones*
Osmolar Concentration
Oxidative Stress / drug effects*,  physiology
Serum Albumin, Bovine / pharmacology
Grant Support
DK47060/DK/NIDDK NIH HHS; R0-1 48452//PHS HHS
Reg. No./Substance:
0/CLU protein, human; 0/Clusterin; 0/Glycoproteins; 0/Irritants; 0/Molecular Chaperones; 0/Serum Albumin, Bovine; 61-82-5/Amitrole; 9001-32-5/Fibrinogen; 97-00-7/Dinitrochlorobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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