Document Detail


A ClpB Chaperone Knockout Mutant of Mesorhizobium ciceri Shows a Delay in the Root Nodulation of Chickpea Plants.
MedLine Citation:
PMID:  23134119     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Several molecular chaperones are known to be involved in bacteria stress response. To investigate the role of chaperone ClpB in rhizobia stress tolerance as well as in the rhizobia-plant symbiosis process, the clpB gene from a chickpea microsymbiont, strain Mesorhizobium ciceri LMS-1, was identified and a knockout mutant was obtained. The ClpB knockout mutant was tested to several abiotic stresses, showing that it was unable to grow after a heat shock and it was more sensitive to acid shock than the wild-type strain. A plant-growth assay performed to evaluate the symbiotic performance of the clpB mutant showed a higher proportion of ineffective root nodules obtained with the mutant than with the wild-type strain. Nodulation kinetics analysis showed a 6- to 8-day delay in nodule appearance in plants inoculated with the ΔclpB mutant. Analysis of nodC gene expression showed lower levels of transcript in the ΔclpB mutant strain. Analysis of histological sections of nodules formed by the clpB mutant showed that most of the nodules presented a low number of bacteroids. No differences in the root infection abilities of green fluorescent protein-tagged clpB mutant and wild-type strains were detected. To our knowledge, this is the first study that presents evidence of the involvement of the chaperone ClpB from rhizobia in the symbiotic nodulation process.
Authors:
Clarisse Brígido; Marta Robledo; Esther Menéndez; Pedro F Mateos; Solange Oliveira
Related Documents :
17881339 - Nodulation of cyclopia spp. (leguminosae, papilionoideae) by burkholderia tuberum.
2997139 - Measurement of the proton motive force in rhizobium meliloti with the escherichia coli ...
24172069 - Routine mapping of fusarium wilt resistance in bc1 populations of arabidopsis thaliana.
16346589 - Invalidity of the concept of slow growth and alkali production in cowpea rhizobia.
24565999 - Cloning of the genome of a goose parvovirus vaccine strain syg61v and rescue of infecti...
23163209 - Virulence genes profiles and phylogenetic origin of escherichia coli from acute and chr...
8842259 - Nosocomial and community-acquired staphylococcus aureus bacteremias from 1980 to 1993: ...
18499359 - Brucella suis identification and biovar typing by real-time pcr.
22180609 - Macellibacteroides fermentans gen. nov., sp. nov., a novel member of the family porphyr...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  25     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1594-604     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Robot-Assisted Intracorporeal Formation of the Ileal Neobladder.
Next Document:  Discovery and Pharmacological Evaluation of a Diphenethylamine Derivative (HS665), a Highly Potent a...