| Clot formation in canine whole blood as measured by rotational thromboelastometry is influenced by sample handling and coagulation activator. | |
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MedLine Citation:
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PMID: 20739876 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The objective of the present study was to systematically evaluate the impact of methodology on thromboelastometry with canine whole blood. Thromboelastometry was performed on citrated blood using a variety of combinations of clotting activators [ex-tem (tissue factor or TF), in-tem (ellagic acid), diluted TF from Innovin, or Ca (recalcification only)] and storage times. Thromboelastometry was also performed using diluted TF from Innovin on blood collected into a contact inhibitor. Ex-vivo contact activation was compared between canine and human blood. Clotting activator had a marked impact on coagulation time, a minor impact on alpha angle, and no impact on clot formation time or maximum clot firmness. When ex-tem or in-tem was the clotting activator, sample storage up to 30 min did not affect results. With diluted TF from Innovin or Ca, sample storage was associated with the development of increased coagulability (as indicated by shorter coagulation time and clot formation time and higher alpha angle) due to ex-vivo contact activation. Canine blood underwent markedly more ex-vivo contact activation than did human blood. Canine blood undergoes significant ex-vivo contact activation during and after collection, which influences thromboelastometry results when a weak clotting activator (such as low TF or recalcification) is used. Thromboelastometry with a strong activator (such as ex-tem or in-tem) is less influenced by ex-vivo changes, and, therefore, likely to be more reflective of in-vivo hemostatic capabilities and to provide consistently interpretable and comparable results. |
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Authors:
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Stephanie A Smith; Maureen McMichael; Alyssa Galligan; Shir Gilor; Crystal M Hoh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis Volume: 21 ISSN: 1473-5733 ISO Abbreviation: Blood Coagul. Fibrinolysis Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9102551 Medline TA: Blood Coagul Fibrinolysis Country: England |
Other Details:
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Languages: eng Pagination: 692-702 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, College of Medicine, University of Illinois at Urbana-Champaign, Urbana-Champaign, Illinois 61801, USA. sasmith6@illinois.edu |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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