| Closure of supporting cell scar formations requires dynamic actin mechanisms. | |
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MedLine Citation:
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PMID: 17716843 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In many vertebrate inner ear sensory epithelia, dying sensory hair cells are extruded, and the apices of surrounding supporting cells converge to re-seal the epithelial barrier between the electrochemically-distinct endolymph and perilymph. These cellular mechanisms remain poorly understood. Dynamic microtubular mechanisms have been proposed for hair cell extrusion; while contractile actomyosin-based mechanisms are required for cellular extrusion and closure in epithelial monolayers. The hypothesis that cytoskeletal mechanisms are required for hair cell extrusion and supporting cell scar formation was tested using bullfrog saccules incubated with gentamicin (6h), and allowed to recover (18h). Explants were then fixed, labeled for actin and cytokeratins, and viewed with confocal microscopy. To block dynamic cytoskeletal processes, disruption agents for microtubules (colchicine, paclitaxel) myosin (Y-27632, ML-9) or actin (cytochalasin D, latrunculin A) were added during treatment and recovery. Microtubule disruption agents had no effect on hair cell extrusion or supporting cell scar formation. Myosin disruption agents appeared to slow down scar formation but not hair cell extrusion. Actin disruption agents blocked scar formation, and largely prevented hair cell extrusion. These data suggest that actin-based cytoskeletal processes are required for hair cell extrusion and supporting cell scar formation in bullfrog saccules. |
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Authors:
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Andrew J Hordichok; Peter S Steyger |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-06-27 |
Journal Detail:
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Title: Hearing research Volume: 232 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-17 Completed Date: 2007-11-20 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7900445 Medline TA: Hear Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-19 Citation Subset: IM |
Affiliation:
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Oregon Hearing Research Center, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism* Amides / pharmacology Animals Anti-Bacterial Agents / toxicity Azepines / pharmacology Bicyclo Compounds, Heterocyclic / pharmacology Cell Death Cicatrix / metabolism*, pathology, physiopathology Colchicine / pharmacology Cytochalasin D / pharmacology Gentamicins / toxicity Hair Cells, Auditory / drug effects*, metabolism, ultrastructure Immunohistochemistry Keratins / metabolism Microscopy, Confocal Microscopy, Electron, Transmission Microtubules / drug effects, metabolism Myosins / metabolism Paclitaxel / pharmacology Pyridines / pharmacology Rana catesbeiana Saccule and Utricle / drug effects*, metabolism, physiopathology, ultrastructure Thiazolidines / pharmacology Time Factors Tissue Culture Techniques Tubulin Modulators / pharmacology* Wound Healing / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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P30 DC05983/DC/NIDCD NIH HHS; R01 DC004555-01A1S1/DC/NIDCD NIH HHS; R01 DC004555-02/DC/NIDCD NIH HHS; R01 DC004555-03/DC/NIDCD NIH HHS; R01 DC004555-05/DC/NIDCD NIH HHS; R01 DC04555/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Amides; 0/Anti-Bacterial Agents; 0/Azepines; 0/Bicyclo Compounds, Heterocyclic; 0/Gentamicins; 0/Pyridines; 0/Thiazolidines; 0/Tubulin Modulators; 105637-50-1/ML 9; 138381-45-0/Y 27632; 22144-77-0/Cytochalasin D; 33069-62-4/Paclitaxel; 64-86-8/Colchicine; 68238-35-7/Keratins; 76343-93-6/latrunculin A; EC 3.6.4.1/Myosins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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