Document Detail


Clostridium saccharogumia sp. nov. and Lactonifactor longoviformis gen. nov., sp. nov., two novel human faecal bacteria involved in the conversion of the dietary phytoestrogen secoisolariciresinol diglucoside.
MedLine Citation:
PMID:  17196483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two anaerobic bacteria involved in the conversion of the plant lignan secoisolariciresinol diglucoside were isolated from faeces of a healthy male adult. The first isolate, strain SDG-Mt85-3Db, was a mesophilic strictly anaerobic Gram-positive helically coiled rod. Based on 16S r RNA gene sequence analysis, its nearest relatives were Clostridium cocleatum (96.7% similarity) and Clostridium ramosum (96.6%). In contrast to these species, the isolate was devoid of alpha-galactosidase and -glucosidase and did not grow on maltose, melibiose, raffinose, rhamnose and trehalose. The hypothesis that strain SDG-Mt85-3Db represents a new bacterial species of the Clostridium cluster XVIII was confirmed by DNA-DNA hybridisation experiments. The G+C content of DNA of strain SDG-Mt85-3Db (30.7+/-0.8 mol%) was comparable with that of Clostridium butyricum, the type species of the genus Clostridium. The name Clostridium saccharogumia is proposed for strain SDG-Mt85-3Db (=DSM 17460T=CCUG 51486T). The second isolate, strain ED-Mt61/PYG-s6, was a mesophilic strictly anaerobic Gram-positive regular rod. Based on 16S rRNA gene sequence analysis, its nearest relatives were Clostridium amygdalinum (93.3%), Clostridium saccharolyticum (93.1%) and Ruminococcus productus (93.0%). The isolate differed from these species in its ability to dehydrogenate enterodiol. It also possessed alpha-arabinosidase and -galactosidase and had a higher G+C content of DNA (48.0 mol%). According to these findings, it is proposed to create a novel genus, Lactonifactor, and a novel species, Lactonifactor longoviformis, to accommodate strain ED-Mt61/PYG-s6. The type strain is DSM 17459T (=CCUG 51487T).
Authors:
Thomas Clavel; Ramona Lippman; Françoise Gavini; Joël Doré; Michael Blaut
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Systematic and applied microbiology     Volume:  30     ISSN:  0723-2020     ISO Abbreviation:  Syst. Appl. Microbiol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-02-20     Completed Date:  2007-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306133     Medline TA:  Syst Appl Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  16-26     Citation Subset:  IM    
Affiliation:
Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbrücke, Arthur-Scheunert-Allee 155, 14558 Nuthetal, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ100445;  DQ100449
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MeSH Terms
Descriptor/Qualifier:
4-Butyrolactone / analogs & derivatives,  metabolism
Adult
Base Composition
Butylene Glycols / metabolism*
Clostridium / classification*,  genetics,  growth & development,  metabolism
Colon / microbiology
Culture Media
DNA / chemistry
DNA, Ribosomal / genetics
Dietary Carbohydrates / metabolism*
Feces / microbiology*
Genotype
Glucosides / metabolism*
Gram-Positive Rods / classification*,  genetics,  growth & development,  metabolism
Humans
Lignans / metabolism
Male
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
Phytoestrogens / metabolism*
RNA, Ribosomal, 16S / genetics
Chemical
Reg. No./Substance:
0/Butylene Glycols; 0/Culture Media; 0/DNA, Ribosomal; 0/Dietary Carbohydrates; 0/Glucosides; 0/Lignans; 0/Phytoestrogens; 0/RNA, Ribosomal, 16S; 148244-82-0/secoisolariciresinol diglucoside; 76543-15-2/2,3-bis(3'-hydroxybenzyl)butyrolactone; 9007-49-2/DNA; 96-48-0/4-Butyrolactone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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