Document Detail


Clostridium perfringens epsilon toxin is cytotoxic for human renal tubular epithelial cells.
MedLine Citation:
PMID:  20488848     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Clostridium perfringens epsilon toxin (ETX) is responsible for a fatal enterotoxemia in different animal species, producing extensive renal damage, neurological disturbance and edema of lungs, heart and kidneys. However, there is no information about the susceptibility of humans to ETX. Here, we report that primary cultures of human renal tubular epithelial cells (HRTEC) exposed to ETX showed a marked swelling with subsequent large blebs surrounding most cells. The incubation of HRTEC with ETX produced a reduction of cell viability in a dose- and time-dependent manner. The CD(50) after 1-hour and 24-hour incubation were 3 µg/mL and 0.5 µg/mL, respectively. The pulse with ETX for 3 min was enough to produce a significant cytotoxic effect on HRTEC after 1-hour incubation. ETX binds to HRTEC forming a large complex of about 160 kDa similar to what was found in the Madin-Darby canine kidney (MDCK) cell line. The HRTEC could be a useful cell model to improve the understanding of the mechanisms involved on the cell damage mediated by ETX.
Authors:
Mariano E Fernandez Miyakawa; Osvaldo Zabal; Claudia Silberstein
Related Documents :
17313178 - Peculiar binding of botulinum neurotoxins.
16451178 - Enterobacter sp. vkgh12 growing with n-butanol as the sole carbon source and cells to w...
10861248 - Inhibition of ca(2+) signalling by p130, a phospholipase-c-related catalytically inacti...
20206198 - Cytotoxic effect of palytoxin on mussel.
16087878 - Carbon nanotubes as multifunctional biological transporters and near-infrared agents fo...
21830188 - Cytotoxic angucyclines from amycolatopsis sp. hca1, a rare actinobacteria derived from ...
Publication Detail:
Type:  Journal Article     Date:  2010-05-20
Journal Detail:
Title:  Human & experimental toxicology     Volume:  30     ISSN:  1477-0903     ISO Abbreviation:  Hum Exp Toxicol     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004560     Medline TA:  Hum Exp Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  275-82     Citation Subset:  IM    
Affiliation:
Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A new sighting study for the fixed concentration procedure to allow for gender differences.
Next Document:  Tricyclic antidepressants intoxication in Tehran, Iran: epidemiology and associated factors.