Document Detail


Clostridium difficile toxins facilitate bacterial colonization by modulating the fence and gate function of colonic epithelium.
MedLine Citation:
PMID:  24273043     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The contribution of Clostridium difficile toxins to cellular intoxication has been extensively studied, but their impact on colonization remains unclear. By setting-up two- and three-dimensional in vitro models of polarized gut epithelium, we investigated how C. difficile infection is affected by host cell polarity and whether TcdA and TcdB contribute to such events. Indeed, we observed that C. difficile adhesion and penetration of the mucosal barrier is substantially enhanced in poorly polarized or EGTA-treated cells, indicating that bacteria bind preferentially to the basolateral cell surface. In this context, we demonstrated that sub-lethal concentrations of C. difficile TcdA are able to alter cell polarity by causing redistribution of plasma membrane components between distinct surface domains. Taken together, the data suggest that toxin-mediated modulation of host cell organization may account for the capacity of this opportunistic pathogen to gain access to basolateral receptors leading to a successful colonization of the colonic mucosa.
Authors:
Magdalena Kasendra; Riccardo Barrile; Rosanna Leuzzi; Marco Soriani
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-11-22
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  -     ISSN:  1537-6613     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy.
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