Document Detail

Closer association of mitochondria with lipid droplets in hepatocytes and activation of Kupffer cells in resveratrol-treated senescence-accelerated mice.
MedLine Citation:
PMID:  21818579     Owner:  NLM     Status:  MEDLINE    
Resveratrol has been extensively investigated because of its beneficial effects in delaying age-related diseases, thus extending the lifespan, possibly by mimicking calorie restriction. For this study, cell biological techniques were used to examine how resveratrol influenced hepatocytes in a senescence-accelerated mouse P10 (SAMP10), treated from 35 to 55 weeks of age, with special emphasis on the relationship between mitochondria and lipid droplets. Survival ratio, body weight and food intake of SAMP10 did not differ significantly between the control and resveratrol-treated groups. Compared with the control, the treated livers were altered significantly, as follows. Lipid droplets were reduced and mitochondria were increased in number in hepatocytes. Phosphorylation of acetyl-CoA carboxylase and the expression of both the mitochondrial ATP synthase β subunit and Mn superoxide dismutase (SOD2) were increased. Mitochondria, expressing more SOD2, were more tightly associated with lipid droplets, suggesting the enhancement of lipolysis through the activation of mitochondrial functions. Cathepsin D expression was less in hepatocytes but enhanced in Kupffer cells, which were increased in number and size with more numerous lysosome-related profiles. Together, resveratrol may activate mitochondria resulting in consuming lipids, and may also activate Kupffer cells by which a beneficial milieu for hepatocytes may be created. Both might be related to improvement in the functioning of the liver, which is the organ that is central to metabolic regulation.
Motoko Shiozaki; Naoya Hayakawa; Masahiro Shibata; Masato Koike; Yasuo Uchiyama; Takahiro Gotow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-05
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  136     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-16     Completed Date:  2012-05-18     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  475-89     Citation Subset:  IM    
Laboratory of Cell Biology, College of Nutrition, Koshien University, Takarazuka, Hyogo, Japan.
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MeSH Terms
Hepatocytes / drug effects*,  metabolism,  pathology
Kupffer Cells / drug effects*,  metabolism,  pathology
Lipids / chemistry*
Mice, Transgenic
Mitochondria / metabolism*
Stilbenes / pharmacology*
Reg. No./Substance:
0/Lipids; 0/Stilbenes; Q369O8926L/resveratrol

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