Document Detail


Close vicinity of Lhc b1 and Lhc b4 in Photosystem II--membrane fragments as verified by chemical cross-linking.
MedLine Citation:
PMID:  9839947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nearest neighbourhood of pigment-protein complexes within Photosystem II (PSII) membrane fragments has been studied by means of chemical cross-linking with o-phthalaldehyde (OPA) in conjunction with protein-chemical techniques. By means of OPA-induced cross-linking a major conjugate of about 60 kDa has been identified. This conjugate was shown to consist of two pigment-protein complexes of light-harvesting complex II (LHC II), Lhc b1 (CP27) and Lhc b4 (CP29) by means of SDS/PAGE in combination with an immunological analysis using mAbs directed against Lhc b4 and by matrix-assisted-laser-desorption-ionization mass spectrometry (MALDI-MS) and sequence analysis of peptides derived from a proteolytic digest of the conjugate. Domains of Lhc bl and Lhc b4 have been localized to a distance of not more than 5 A within LHC II. Our results are discussed in the light of recent models on the topography of the two subunits within the antenna system of Photosystem II.
Authors:
J Miao; K D Irrgang; J Salnikow; P Franke; J Vater
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  257     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-22     Completed Date:  1998-12-22     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  586-91     Citation Subset:  IM    
Affiliation:
Technische Universität Berlin, Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cross-Linking Reagents / chemistry
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Photosynthetic Reaction Center Complex Proteins / chemistry*
Photosystem II Protein Complex
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spinacia oleracea
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Photosynthetic Reaction Center Complex Proteins; 0/Photosystem II Protein Complex

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