| Clopidogrel Effectively Suppresses Endothelial Microparticle Generation Induced by Indoxyl Sulfate via Inhibition of the p38 Mitogen-Activated Protein Kinase Pathway. | |
| | |
MedLine Citation:
|
PMID: 21811066    Owner: NLM    Status: Publisher    |
Abstract/OtherAbstract:
|
Background/Aims: Endothelial microparticles (EMPs) are closely associated with vascular dysfunction. We investigated the effects of several drugs on EMP generation in human umbilical vein endothelial cells (HUVECs), and the involvement of the mitogen-activated protein kinase (MAPK) in EMP generation. Methods: CD31+CD42-EMP counts were measured by flow cytometry in supernatants of HUVECs incubated with indoxyl sulfate. The EMP responses to losartan, lovastatin, clopidogrel, and mesoglycan were examined. We then measured the effects of MAPK inhibitors on EMPs. Results: (1) Indoxyl sulfate induced EMP release in HUVECs in a dose-dependent fashion; (2) all drugs (10-50 μM) inhibited EMP generation induced by indoxyl sulfate, with clopidogrel being the most effective; (3) the p38 MAPK inhibitor suppressed EMP generation induced by indoxyl sulfate, and (4) clopidogrel significantly suppressed MAPK signaling activated by indoxyl sulfate, with the most potency on p38. Conclusion: The p38 signaling involves EMP generation induced by indoxyl sulfate and is effectively suppressed by clopidogrel. |
| | |
Authors:
|
Jung-Hwa Ryu; Seung-Jung Kim |
Related Documents
:
|
21868386Â -Â Involvement of 14-3-3 proteins in the second epidermal growth factor-induced wave of ra... 21040116Â -Â Carbon monoxide has antioxidative properties in the liver involving p38 map kinase path... 22199356Â -Â C-abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase Î... 10217536Â -Â Induction of cyclo-oxygenase-2 expression by methyl arachidonyl fluorophosphonate in mu... 8660676Â -Â Purification and characterization of protein phosphatase 2c in rat parotid acinar cells... 16565316Â -Â Heat shock-induced cardioprotection activates cytoskeletal-based cell survival pathways. |
Publication Detail:
|
Type:Â JOURNAL ARTICLE Â Â Â Date:Â 2011-7-29 |
Journal Detail:
|
Title: Blood purification    Volume: 32    ISSN: 1421-9735    ISO Abbreviation: -    Publication Date: 2011 Jul |
Date Detail:
|
Created Date:Â 2011-8-3 Â Â Â Completed Date:Â - Â Â Â Revised Date:Â - Â Â Â |
Medline Journal Info:
|
Nlm Unique ID: 8402040    Medline TA: Blood Purif    Country: -    |
Other Details:
|
Languages:Â ENG Â Â Â Pagination:Â 186-194 Â Â Â Citation Subset:Â - Â Â Â |
Copyright Information:
|
Copyright © 2011 S. Karger AG, Basel. |
Affiliation:
|
Division of Nephrology, Department of Internal Medicine, Ewha Womans University School of Medicine, Seoul, Korea. |
Export Citation:
|
APA/MLA Format    Download EndNote    Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Does Antifungal Prophylaxis with Daily Oral Fluconazole Reduce the Risk of Fungal Peritonitis in Per...
Next Document: Measurement of Vascular Access Flow by Online Clearance Monitor in Online Hemodiafiltration.