Document Detail


Clonogenicity of mammalian cells in hybrid spheroids: a new assay method.
MedLine Citation:
PMID:  2305028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new in vitro method for determining the clonogenicity of mammalian cells in culture is described. The method is based on packaging clonogens into agglomerates of non-proliferating, but metabolically active, HeLa cells. These agglomerates, termed hybrid spheroids, provide an in vivo-like environment for entrapped test cells, offering a realistic system for prospective tumor control studies. Clonogenicity is determined by varying the number of test cells per hybrid spheroid so that some, but not all, spheroids give rise to macrocolonies. From the fraction of non-colony forming spheroids, the average number of clonogens per spheroid can be calculated, and the survival of irradiated test cells determined. In this fashion survival curves were obtained for HeLa, B-16 and HEp3 cells which corresponded to survival curves obtained in the conventional manner. The clonogenicity of cells, derived from a human maxillary melanoma surgical specimen was also determined by the hybrid spheroid method. With this method, plating efficiency increased in those cells which normally plate poorly, such as tumor cells, thus enabling survival measurements when this is not practical using conventional methods.
Authors:
B Djordjevic; C S Lange
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Radiation and environmental biophysics     Volume:  29     ISSN:  0301-634X     ISO Abbreviation:  Radiat Environ Biophys     Publication Date:  1990  
Date Detail:
Created Date:  1990-03-23     Completed Date:  1990-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0415677     Medline TA:  Radiat Environ Biophys     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  31-46     Citation Subset:  IM    
Affiliation:
Department of Radiation Oncology, SUNY Health Sciences Center, Brooklyn 11203.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Clone Cells / pathology*
Dose-Response Relationship, Radiation
Hela Cells
Histocytochemistry
Humans
Hybrid Cells / pathology,  radiation effects
Mice
Organoids
Tumor Cells, Cultured / pathology*
Tumor Stem Cell Assay
Grant Support
ID/Acronym/Agency:
R01 CA 35289/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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