Document Detail

Cloning of a unique sequence specific to isolates of type B:2 Pasteurella multocida.
MedLine Citation:
PMID:  8938847     Owner:  NLM     Status:  MEDLINE    
Two Carter type B Pasteurella multocida isolates, Izatnagar 52 and 25, isolated from cases of haemorrhagic septicaemia (HS), were used in a modified subtractive hybridisation technique with the specific aim of cloning unique DNA sequences related to the pathogenesis of HS. Biochemical and protein analyses have shown these isolates to be similar, but reports indicate that they have differences in pathogenicity. The subtracted inserts were screened against genomic DNA from a wide range of P multocida isolates, with two distinct fragments demonstrating specific hybridisation with Carter type B isolates that cause HS. No identity was observed with either Carter type E isolates or non-HS type B strains. The clones were sequenced and a search of the GenBank database revealed significant identity of the clone A3b (296 nt) to P haemolytica lipoprotein, whereas there was no significant identity with 6b (956 nt). Both these fragments had a high level of identity (72.8 to 76.9 per cent) to the H influenzae Rd genome.
K M Townsend; H J Dawkins; B J Zeng; M W Watson; J M Papadimitriou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Research in veterinary science     Volume:  61     ISSN:  0034-5288     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-02-28     Completed Date:  1997-02-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  199-205     Citation Subset:  IM    
Department of Pathology, University of Western Australia.
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MeSH Terms
Base Sequence
Cattle Diseases
Cloning, Molecular
DNA, Bacterial / analysis
Genome, Bacterial
Molecular Sequence Data
Open Reading Frames
Pasteurella Infections / microbiology,  veterinary*
Pasteurella multocida / classification*,  genetics*,  isolation & purification
Poultry Diseases
Sheep Diseases
Swine Diseases
Terminator Regions, Genetic
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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