| Cloning and sequencing of a poly(DL-lactic acid) depolymerase gene from Paenibacillus amylolyticus strain TB-13 and its functional expression in Escherichia coli. | |
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MedLine Citation:
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PMID: 12732514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gene encoding a poly(DL-lactic acid) (PLA) depolymerase from Paenibacillus amylolyticus strain TB-13 was cloned and overexpressed in Escherichia coli. The purified recombinant PLA depolymerase, PlaA, exhibited degradation activities toward various biodegradable polyesters, such as poly(butylene succinate), poly(butylene succinate-co-adipate), poly(ethylene succinate), and poly(epsilon-caprolactone), as well as PLA. The monomeric lactic acid was detected as the degradation product of PLA. The substrate specificity toward triglycerides and p-nitrophenyl esters indicated that PlaA is a type of lipase. The gene encoded 201 amino acid residues, including the conserved pentapeptide Ala-His-Ser-Met-Gly, present in the lipases of mesophilic Bacillus species. The identity of the amino acid sequence of PlaA with Bacillus lipases was no more than 45 to 50%, and some of its properties were different from those of these lipases. |
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Authors:
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Yukie Akutsu-Shigeno; Teerawat Teeraphatpornchai; Kamonluck Teamtisong; Nobuhiko Nomura; Hiroo Uchiyama; Tadaatsu Nakahara; Toshiaki Nakajima-Kambe |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 69 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-06 Completed Date: 2003-08-05 Revised Date: 2010-10-22 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2498-504 Citation Subset: IM |
Affiliation:
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PRESTO, Japan Science and Technology Corporation, Tsukuba, Ibaraki 305-8572, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB093482 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacillus / enzymology, genetics Bacteria / enzymology*, genetics* Base Sequence Biodegradation, Environmental Carboxylic Ester Hydrolases / genetics*, metabolism Cloning, Molecular Conserved Sequence DNA, Bacterial / genetics Enzyme Stability Escherichia coli / genetics Gene Expression Genes, Bacterial* Kinetics Lipase / genetics Molecular Sequence Data Polyesters / metabolism Recombinant Proteins / genetics, metabolism Sequence Homology, Amino Acid Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Polyesters; 0/Recombinant Proteins; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/poly(lactic acid) depolymerase; EC 3.1.1.3/Lipase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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