Document Detail

Cloning and sequencing of the ilvBNC gene cluster from Mycobacterium avium.
MedLine Citation:
PMID:  8921849     Owner:  NLM     Status:  MEDLINE    
Disseminated Mycobacterium avium (Ma)-M. intracellulare disease is a prevalent opportunistic infection in patients with Acquired Immune Deficiency Syndrome. Mycobacteria produce a variety of fatty acids which provide the first line of defence against potentially lethal environmental conditions. The metabolism of the branched-chain amino acids (BCAA) could be correlated to the production of branched-chain fatty acids in mycobacteria. In order to develop a better understanding of Mycobacterium BCAA biosynthesis, three genes, ilvBN and ilvC, encoding acetohydroxy acid synthase (AHS) and acetohydroxy acid isomeroreductase (IR), respectively, were cloned from Ma. The genes were isolated by screening a Ma genomic library with a heterologous probe. The deduced amino acid sequences revealed significant homology to the AHS and IR proteins from other bacterial species.
L Gusberti; R Cantoni; E De Rossi; M Branzoni; G Riccardi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  177     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-26     Completed Date:  1996-12-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  83-5     Citation Subset:  IM    
Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Università di Pavia, Italy.
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MeSH Terms
Acetolactate Synthase / genetics*
Alcohol Oxidoreductases / genetics*
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Bacterial
Ketol-Acid Reductoisomerase
Molecular Sequence Data
Multigene Family*
Mycobacterium avium / enzymology,  genetics*
Reg. No./Substance:
0/DNA, Bacterial; EC 1.1.-/Alcohol Oxidoreductases; EC Reductoisomerase; EC Synthase

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