Document Detail

Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein.
MedLine Citation:
PMID:  1353107     Owner:  NLM     Status:  MEDLINE    
The prototype Puumala virus (PV) Sotkamo strain small (S) and medium (M) RNA genome segments were amplified by the polymerase chain reaction (PCR), cloned and sequenced. The S segment is 1830 nucleotides long with an open reading frame coding for 433 amino acids. The identity to the PV Hällnäs strain was 83% at the nucleotide and 96% at the amino acid level. The M segment in the Sotkamo strain is 3616 nucleotides long and contains one open reading frame of 1148 amino acids with 83% nucleotide and 94% amino acid identity to the Hällnäs strain. Most amino acid changes were conservative and the five predicted glycosylation sites are identical. The amino acid identity to the prototype hantavirus, Hantaan virus, was 62 and 54% for S and M segments, respectively. The coding region of the S segment was further amplified by PCR, ligated to pEX vectors and expressed in Escherichia coli as a beta-galactosidase fusion protein and was seen to be specifically detected by nephropathia epidemica sera in immunoblotting.
O Vapalahti; H Kallio-Kokko; E M Salonen; M Brummer-Korvenkontio; A Vaheri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  73 ( Pt 4)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-08-21     Completed Date:  1992-08-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  829-38     Citation Subset:  IM    
Department of Virology, University of Helsinki, Finland.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X61034;  X61035
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Western
Cloning, Molecular
Genetic Variation
Hantavirus / genetics*,  isolation & purification
Hemorrhagic Fever with Renal Syndrome / diagnosis*
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Viral / genetics*
Reading Frames / genetics
Recombinant Fusion Proteins / biosynthesis
Sequence Homology, Nucleic Acid
Vero Cells
Viral Proteins / biosynthesis*,  blood,  genetics
beta-Galactosidase / biosynthesis
Reg. No./Substance:
0/RNA, Viral; 0/Recombinant Fusion Proteins; 0/Viral Proteins; EC

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