| Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein. | |
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MedLine Citation:
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PMID: 1353107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The prototype Puumala virus (PV) Sotkamo strain small (S) and medium (M) RNA genome segments were amplified by the polymerase chain reaction (PCR), cloned and sequenced. The S segment is 1830 nucleotides long with an open reading frame coding for 433 amino acids. The identity to the PV Hällnäs strain was 83% at the nucleotide and 96% at the amino acid level. The M segment in the Sotkamo strain is 3616 nucleotides long and contains one open reading frame of 1148 amino acids with 83% nucleotide and 94% amino acid identity to the Hällnäs strain. Most amino acid changes were conservative and the five predicted glycosylation sites are identical. The amino acid identity to the prototype hantavirus, Hantaan virus, was 62 and 54% for S and M segments, respectively. The coding region of the S segment was further amplified by PCR, ligated to pEX vectors and expressed in Escherichia coli as a beta-galactosidase fusion protein and was seen to be specifically detected by nephropathia epidemica sera in immunoblotting. |
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Authors:
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O Vapalahti; H Kallio-Kokko; E M Salonen; M Brummer-Korvenkontio; A Vaheri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 73 ( Pt 4) ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-08-21 Completed Date: 1992-08-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 829-38 Citation Subset: IM |
Affiliation:
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Department of Virology, University of Helsinki, Finland. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/X61034; X61035 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Blotting, Western Cloning, Molecular Genetic Variation Hantavirus / genetics*, isolation & purification Hemorrhagic Fever with Renal Syndrome / diagnosis* Molecular Sequence Data Polymerase Chain Reaction RNA, Viral / genetics* Reading Frames / genetics Recombinant Fusion Proteins / biosynthesis Sequence Homology, Nucleic Acid Vero Cells Viral Proteins / biosynthesis*, blood, genetics beta-Galactosidase / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral; 0/Recombinant Fusion Proteins; 0/Viral Proteins; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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