| Cloning and sequencing of 5' flanking sequence from the gene encoding 2S storage protein, from two Brassica species. | |
| | |
MedLine Citation:
|
PMID: 8224919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Using oligodeoxyribonucleotide primers and the polymerase chain reaction, we have cloned and sequenced about 1.2 kb of upstream sequences from two members of the 2S seed storage protein-encoding gene family from Brassica juncea and B. oleracea. The two sequences bear more than 90% homology and have characteristic seed-specific promoter motifs. The high degree of sequence conservation indicates that this napin-encoding gene family evolved earlier than the divergence of the three primary Brassica species and their amphidiploids, and the sequences have been conserved due to some metabolic constraints in seed development. |
| | |
Authors:
|
S Dasgupta; J Dasgupta; R K Mandal |
Related Documents
:
|
10743659 - Molecular cloning, functional expression and characterisation of rcc reductase involved... 8118059 - Cloning and expression analysis of a viroid-induced peroxidase from tomato plants. 1021549 - Somatic hybridization in higher plants. 16450169 - Characterization and functional analysis of a pollen-specific gene st901 in solanum tub... 16593689 - Genomic and cdna clones for maize phosphoenolpyruvate carboxylase and pyruvate,orthopho... 8541659 - Assessment of the mutagenicity of extracts of tmv-coat-protein-gene induced transgenic ... 2583119 - A u-snrna gene-specific upstream element and a -30 'tata box' are required for transcri... 23672489 - Characterisation of a transcriptome to find sequence differences between two differenti... 7705369 - Molecular cloning of a cdna that encodes the precursor to several exogastrula-inducing ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Gene Volume: 133 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1993 Nov |
Date Detail:
|
Created Date: 1993-12-22 Completed Date: 1993-12-22 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 301-2 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Bose Institute, Calcutta, India. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/L24527; L24528; L24529; X67833; X70333; Z12148; Z12149; Z12150; Z21919; Z21920 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
2S Albumins, Plant Base Sequence Brassica / genetics* Cloning, Molecular Conserved Sequence DNA Molecular Sequence Data Plant Proteins / genetics* Promoter Regions, Genetic Sequence Homology, Nucleic Acid |
| Chemical | |
Reg. No./Substance:
|
0/2S Albumins, Plant; 0/Plant Proteins; 89147-51-3/napin protein, Brassica napus; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sequence of the human lens beta B2-crystallin-encoding cDNA.
Next Document: Sequence of the cDNA encoding an actin homolog in the crayfish Procambarus clarkii.