Document Detail


Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites.
MedLine Citation:
PMID:  23024043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To our knowledge, few reports on Demodex studied at the molecular level are available at present. In this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (CHS) gene fragments of Demodex folliculorum, Demodex brevis, and Demodex canis (three isolates from each species) from Xi'an China, by designing specific primers based on the only partial sequence of the CHS gene of D. canis from Japan, retrieved from GenBank. Results show that amplification was successful only in three D. canis isolates and one D. brevis isolate out of the nine Demodex isolates. The obtained fragments were sequenced to be 339 bp for D. canis and 338 bp for D. brevis. The CHS gene sequence similarities between the three Xi'an D. canis isolates and one Japanese D. canis isolate ranged from 99.7% to 100.0%, and those between four D. canis isolates and one D. brevis isolate were 99.1%-99.4%. Phylogenetic trees based on maximum parsimony (MP) and maximum likelihood (ML) methods shared the same clusters, according with the traditional classification. Two open reading frames (ORFs) were identified in each CHS gene sequenced, and their corresponding amino acid sequences were located at the catalytic domain. The relatively conserved sequences could be deduced to be a CHS class A gene, which is associated with chitin synthesis in the integument of Demodex mites.
Authors:
Ya-e Zhao; Zheng-hang Wang; Yang Xu; Ji-ru Xu; Wen-yan Liu; Meng Wei; Chu-ying Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  13     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-03-04     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  763-8     Citation Subset:  IM    
Affiliation:
Department of Immunology and Pathogen Biology, Xi'an Jiaotong University College of Medicine, Xi'an, China. zhaoyae@mail.xjtu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Chitin Synthase / genetics*
Cloning, Molecular
DNA Primers / genetics
Mites / classification,  enzymology*,  genetics*
Molecular Sequence Data
Open Reading Frames
Phylogeny
Sequence Homology, Nucleic Acid
Species Specificity
Chemical
Reg. No./Substance:
0/DNA Primers; EC 2.4.1.16/Chitin Synthase
Comments/Corrections

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