Document Detail


Cloning, purification and biochemical characterization of a thrombus-ditargeting thrombolytic agent, comprised of annexin B1, ScuPA-32K and fibrin-adherent peptide.
MedLine Citation:
PMID:  16797768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of thrombolytic agent could provide invaluable progress for antithrombotic therapy. In this paper, we reported the cloning, purification and biochemical characterization of AnxB1ScuPAFap, a thrombus-ditargeting chimera composed of annexin B1, low molecular single-chain urokinase (ScuPA-32K) and fibrin-adherent peptide (dodecapeptide, Fap). In vitro test showed that, the chimera was a thrombolytic agent with anticoagulant activity and thrombus-ditargeting with the activated-platelet membrane binding and fibrin clot binding activity. Compared to urokinase, the chimera had less reperfusion time, higher reperfusion ratio, and less bleeding effects on coronary thrombolysis by clot lysis assay in dogs. Thus, the chimera appeared to be suitable for thrombolytic therapy of thrombus diseases.
Authors:
Fei-Xiang Ding; Hong-Li Yan; Yi-Ming Lu; Geng Xue; Qian Mei; Jing-Jing Huang; Zhuan-You Zhao; Yu-Zhao Wang; Shu-Han Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-07
Journal Detail:
Title:  Journal of biotechnology     Volume:  126     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-18     Completed Date:  2006-12-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  394-405     Citation Subset:  IM    
Affiliation:
Department of Medical Genetics, The Second Military Medical University, Xiangyin Road 800, Shanghai 200433, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexins / administration & dosage*,  chemistry,  metabolism*
Blood Coagulation / drug effects
Cloning, Molecular / methods
Coronary Thrombosis / drug therapy*,  pathology
Dogs
Fibrinogen / administration & dosage*,  chemistry,  metabolism*
Fibrinolytic Agents / chemistry,  metabolism,  pharmacology
Helminth Proteins / administration & dosage*,  chemistry,  metabolism*
Male
Peptides / administration & dosage,  chemistry,  metabolism
Recombinant Fusion Proteins / biosynthesis,  chemistry,  pharmacology
Urokinase-Type Plasminogen Activator / administration & dosage*,  chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Annexins; 0/Fibrinolytic Agents; 0/Helminth Proteins; 0/Peptides; 0/Recombinant Fusion Proteins; 0/annexin B1, Taenia solium; 9001-32-5/Fibrinogen; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

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