Document Detail


Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat.
MedLine Citation:
PMID:  23017836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipocalins are a structurally conserved and diversely functional family of proteins that are of potential importance in epididymis functions. The rat Lcn9 gene was cloned by in silico methods and genome walking based on homology to the rhesus monkey epididymal ESC513 and its polyclonal antisera were prepared. The rat Lcn9 gene is located on chromosome 3p13 spanning 7 exons, contains 2.3 kb and encodes 179 amino acids with a 17-amino acid signal peptide. Northern blot, western blot, and immunohistochemical staining analysis revealed that rat Lcn9 was a novel epididymis-specific gene, expressed selectively in the proximal caput region, influenced by luminal fluid testicular factors. Moreover, Lcn9 protein was modified by N-glycosylation and bound on the postacrosomal domain of caput sperm. In conclusion, the rat Lcn9 exhibited tissue-, region-, and temporal-specific expression patterns and its expression was regulated by luminal testicular factors. Its potential roles in sperm maturation are discussed.
Authors:
Xiangqi Li; Xiaoni Zhan; Shigui Liu; Shuanggang Hu; Chunfang Zhu; Susan H Hall; Frank S French; Qiang Liu; Yonglian Zhang
Related Documents :
7896056 - Effects of vasoactive intestinal peptide and its homologues on the noradrenaline-mediat...
25352656 - Involvement of the h+/organic cation antiporter in nicotine transport in rat liver.
3857896 - Sex differences in the effect of extirpation of the submandibular salivary glands in rats.
25448286 - Itraq-based proteomic analysis of combination therapy with taurine, epigallocatechin ga...
5687586 - Effects of guanethidine on the blood pressure response to splanchnic nerve stimulation ...
8738236 - Comparison of succinate dehydrogenase activity and soma size relationships among neuron...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  44     ISSN:  1745-7270     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-27     Revised Date:  2013-10-09    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  876-85     Citation Subset:  IM    
Affiliation:
Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ537496
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Western
Cloning, Molecular
DNA, Complementary / chemistry,  genetics
Epididymis / metabolism*
Gene Expression Profiling*
Gene Expression Regulation / drug effects
Glycosylation
Immunohistochemistry
Lipocalins / genetics*,  metabolism
Male
Molecular Sequence Data
Orchiectomy
Rats
Rats, Sprague-Dawley
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Testosterone Propionate / pharmacology
Time Factors
Grant Support
ID/Acronym/Agency:
2 D43 TW000627-11/TW/FIC NIH HHS; 2 D43 TW000627-12/TW/FIC NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Lcn9 protein, rat; 0/Lipocalins; 57-85-2/Testosterone Propionate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The comprehensive effects of hyperlipidemia and hyperhomocysteinemia on pathogenesis of atherosclero...
Next Document:  Heteroexpression of the wheat phytochelatin synthase gene (TaPCS1) in rice enhances cadmium sensitiv...