Document Detail

Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat.
MedLine Citation:
PMID:  23017836     Owner:  NLM     Status:  MEDLINE    
Lipocalins are a structurally conserved and diversely functional family of proteins that are of potential importance in epididymis functions. The rat Lcn9 gene was cloned by in silico methods and genome walking based on homology to the rhesus monkey epididymal ESC513 and its polyclonal antisera were prepared. The rat Lcn9 gene is located on chromosome 3p13 spanning 7 exons, contains 2.3 kb and encodes 179 amino acids with a 17-amino acid signal peptide. Northern blot, western blot, and immunohistochemical staining analysis revealed that rat Lcn9 was a novel epididymis-specific gene, expressed selectively in the proximal caput region, influenced by luminal fluid testicular factors. Moreover, Lcn9 protein was modified by N-glycosylation and bound on the postacrosomal domain of caput sperm. In conclusion, the rat Lcn9 exhibited tissue-, region-, and temporal-specific expression patterns and its expression was regulated by luminal testicular factors. Its potential roles in sperm maturation are discussed.
Xiangqi Li; Xiaoni Zhan; Shigui Liu; Shuanggang Hu; Chunfang Zhu; Susan H Hall; Frank S French; Qiang Liu; Yonglian Zhang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  44     ISSN:  1745-7270     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-27     Revised Date:  2013-10-09    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  876-85     Citation Subset:  IM    
Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Western
Cloning, Molecular
DNA, Complementary / chemistry,  genetics
Epididymis / metabolism*
Gene Expression Profiling*
Gene Expression Regulation / drug effects
Lipocalins / genetics*,  metabolism
Molecular Sequence Data
Rats, Sprague-Dawley
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Testosterone Propionate / pharmacology
Time Factors
Grant Support
2 D43 TW000627-11/TW/FIC NIH HHS; 2 D43 TW000627-12/TW/FIC NIH HHS
Reg. No./Substance:
0/DNA, Complementary; 0/Lcn9 protein, rat; 0/Lipocalins; 57-85-2/Testosterone Propionate

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