| Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat. | |
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MedLine Citation:
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PMID: 23017836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipocalins are a structurally conserved and diversely functional family of proteins that are of potential importance in epididymis functions. The rat Lcn9 gene was cloned by in silico methods and genome walking based on homology to the rhesus monkey epididymal ESC513 and its polyclonal antisera were prepared. The rat Lcn9 gene is located on chromosome 3p13 spanning 7 exons, contains 2.3 kb and encodes 179 amino acids with a 17-amino acid signal peptide. Northern blot, western blot, and immunohistochemical staining analysis revealed that rat Lcn9 was a novel epididymis-specific gene, expressed selectively in the proximal caput region, influenced by luminal fluid testicular factors. Moreover, Lcn9 protein was modified by N-glycosylation and bound on the postacrosomal domain of caput sperm. In conclusion, the rat Lcn9 exhibited tissue-, region-, and temporal-specific expression patterns and its expression was regulated by luminal testicular factors. Its potential roles in sperm maturation are discussed. |
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Authors:
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Xiangqi Li; Xiaoni Zhan; Shigui Liu; Shuanggang Hu; Chunfang Zhu; Susan H Hall; Frank S French; Qiang Liu; Yonglian Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 44 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-28 Completed Date: 2013-02-27 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 876-85 Citation Subset: IM |
Affiliation:
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Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/DQ537496 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Western Cloning, Molecular DNA, Complementary / chemistry, genetics Epididymis / metabolism* Gene Expression Profiling* Gene Expression Regulation / drug effects Glycosylation Immunohistochemistry Lipocalins / genetics*, metabolism Male Molecular Sequence Data Orchiectomy Rats Rats, Sprague-Dawley Sequence Analysis, DNA Sequence Homology, Amino Acid Testosterone Propionate / pharmacology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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2 D43 TW000627-11/TW/FIC NIH HHS; 2 D43 TW000627-12/TW/FIC NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Lcn9 protein, rat; 0/Lipocalins; 57-85-2/Testosterone Propionate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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