Document Detail

Cloning and polymerase chain reaction-single-strand conformation polymorphism analysis of anonymous Alu repeats on chromosome 11.
MedLine Citation:
PMID:  1733851     Owner:  NLM     Status:  MEDLINE    
We have shown that many of the Alu repeats found in the GenBank database are polymorphic and that this polymorphism can be detected by a simple technique, single-strand conformation polymorphism (SSCP) analysis, after polymerase chain reaction (PCR) amplification of each repeat from DNA of individuals. Here, we describe a method for collecting many anonymous Alu repeats and their flanks in a chromosome-specific phage library and cloning them into plasmids. The flanking single-copy sequences of each repeat in the plasmid were then determined, and 20mer to 30mer segments of these sequences were used as primers for the PCR-SSCP analysis. Many new polymorphic DNA markers on chromosome 11 were obtained with this method. These markers can also serve as sequence-tagged sites for physical mapping of the genome.
M Iizuka; S Mashiyama; M Oshimura; T Sekiya; K Hayashi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  12     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-03-05     Completed Date:  1992-03-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  139-46     Citation Subset:  IM    
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
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MeSH Terms
Bacteriophages / genetics
Base Sequence
Chromosomes, Human, Pair 11*
Cloning, Molecular
DNA, Single-Stranded / chemistry*
Genetic Vectors
Genomic Library
Molecular Sequence Data
Nucleic Acid Conformation
Polymerase Chain Reaction / methods*
Polymorphism, Genetic*
Repetitive Sequences, Nucleic Acid*
Reg. No./Substance:
0/DNA, Single-Stranded

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