Document Detail

Cloning and partial characterization of the xanthine dehydrogenase gene of Calliphora vicina, a distant relative of Drosophila melanogaster.
MedLine Citation:
PMID:  2830167     Owner:  NLM     Status:  MEDLINE    
In vitro enzymatic assays have shown that an enzyme with typical xanthine dehydrogenase (XDH) activities and electrophoretic mobility slightly different from that of Drosophila XDH is present in Calliphora tissues. A Calliphora genomic sequence has been isolated by low-stringency hybridization to the Drosophila rosy gene (XDH), and partially sequenced. This sequence has been shown to be unique, polymorphic, and it maps on chromosome I. Sequence comparisons provide compelling evidence that it belongs to the XDH gene of Calliphora. Interspecies transformation experiments, aimed at investigating functional as well as structural divergence of the XDH genes of Calliphora and Drosophila, are now possible.
C Rocher-Chambonnet; P Berreur; M Houde; M C Tiveron; J A Lepesant; F Brégégère
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  59     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1987  
Date Detail:
Created Date:  1988-04-04     Completed Date:  1988-04-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  201-12     Citation Subset:  IM    
Institut Jacques Monod, Université Paris, France.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular*
DNA Restriction Enzymes
Diptera / enzymology,  genetics*
Drosophila melanogaster / cytology,  genetics*
Ketone Oxidoreductases / genetics*
Molecular Sequence Data
Nucleic Acid Hybridization
Xanthine Dehydrogenase / genetics*
Reg. No./Substance:
EC Dehydrogenase; EC 1.2.-/Ketone Oxidoreductases; EC 3.1.21.-/DNA Restriction Enzymes

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