| Cloning and nucleotide sequence of the structural gene encoding for human tryptophanyl-tRNA synthetase. | |
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MedLine Citation:
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PMID: 1765274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A structural gene encoding bovine (b) tryptophanyl-tRNA synthetase (WRS) has recently been cloned and sequenced [Garret et al., Biochemistry 30 (1991) 7809-7817]. Using part of this sequence as a hybridisation probe we have cloned and sequenced a structural gene encoding human polypeptide highly homologous with two mammalian proteins, bWRS [Garret et al., Biochemistry 30 (1991) 7809-7817; EMBL accession No. X52113] and rabbit peptide chain release factor [Lee et al., Proc. Natl. Acad. Sci. USA 87 (1990) 3508-3512]. Identification of the sequence encoding a human WRS is based on (i) the presence of 'HIGH' and 'KMSKS' structural motifs typical for class-I aminoacyl-tRNA synthetases [Eriani et al., Nature 347 (1990) 203-206]; (ii) coincidence of the number of SH groups per subunit estimated experimentally [Muench et al., Science 187 (1975) 1089-1091] and deduced from the cDNA sequence (six in both cases); (iii) close resemblance of two WRS polypeptides sequenced earlier [Muench et al., Science 187 (1975) 1089-1091] and the predicted structure in two different regions. |
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Authors:
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Frolova LYu; M A Sudomoina; Grigorieva AYu; O L Zinovieva; L L Kisselev |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gene Volume: 109 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-02-18 Completed Date: 1992-02-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 291-6 Citation Subset: IM |
Affiliation:
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Engelhardt Institute of Molecular Biology, U.S.S.R. Academy of Sciences, Moscow. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M61715; S75821; S75861; S75864; S75866; S75868; S75872; S75874; S75876; S75878 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular Humans Molecular Sequence Data Open Reading Frames / genetics RNA, Transfer, Trp / metabolism* Restriction Mapping Sequence Alignment Sequence Homology, Nucleic Acid Tryptophan-tRNA Ligase / genetics* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Transfer, Trp; EC 6.1.1.2/Tryptophan-tRNA Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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