Document Detail


Cloning of a novel monoamine oxidase cDNA from trout liver.
MedLine Citation:
PMID:  7808446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A trout liver monoamine oxidase (MAO) cDNA was cloned by screening a cDNA library with a human MAO-A cDNA probe. The trout MAO cDNA encodes 499 amino acids, with a molecular mass of 56.6 kDa. The deduced amino acid sequence of trout MAO shows 70% and 71% identity with those of human MAO-A and MAO-B, respectively. Trout MAO contains the pentapeptide sequence Ser-Gly-Gly-Cys-Tyr, to which the cofactor FAD is covalently bound. Transient expression of the cDNA in COS-7 cells shows that trout MAO oxidizes both serotonin [5-hydroxytryptamine (5-HT)] and beta-phenylethylamine (PEA), unlike human MAO-A and MAO-B, which oxidize only 5-HT and PEA, respectively. The Km for 5-HT is similar for trout MAO (130 +/- 17 mM) and human MAO-A (68 +/- 4 mM). The Km for PEA is similar for trout MAO (12.5 +/- 2.0 mM) and human MAO-B (1.5 +/- 0.2 mM). When 5-HT is used as a substrate, trout MAO is more sensitive to clorgyline (IC50, 2.8 +/- 0.2 x 10(-8) M) than deprenyl (IC50, 1.0 +/- 0.1 x 10(-6) M), a result similar to the inhibition selectivity of human MAO-A. However, trout MAO is less sensitive to clorgyline than is human MAO-A (IC50, 5.8 +/- 0.1 x 10(-10) M). Trout MAO is less sensitive to deprenyl (IC50, 4.6 +/- 0.3 x 10(-7) M) than is human MAO-B (IC50, 1.4 +/- 0.1 x 10(-9) M) when PEA is used as the substrate. These results indicate that trout MAO displays substrate and inhibitor selectivities that are not identical to those of either MAO-A and -B, and it therefore represents a novel type of MAO. The structure of trout MAO will provide insights into the substrate and inhibitor selectivities of the MAOs.
Authors:
K Chen; H F Wu; J Grimsby; J C Shih
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular pharmacology     Volume:  46     ISSN:  0026-895X     ISO Abbreviation:  Mol. Pharmacol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-02-02     Completed Date:  1995-02-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0035623     Medline TA:  Mol Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1226-33     Citation Subset:  IM    
Affiliation:
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles 90033.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L37878
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cloning, Molecular
DNA, Complementary
Humans
Liver / enzymology*
Molecular Sequence Data
Monoamine Oxidase / genetics*
Monoamine Oxidase Inhibitors / pharmacology
Sequence Homology, Amino Acid
Trout
Grant Support
ID/Acronym/Agency:
K05-MH00796/MH/NIMH NIH HHS; R01-MH37020/MH/NIMH NIH HHS; R37-MH39085/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Monoamine Oxidase Inhibitors; EC 1.4.3.4/Monoamine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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