| Cloning of a novel monoamine oxidase cDNA from trout liver. | |
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MedLine Citation:
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PMID: 7808446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A trout liver monoamine oxidase (MAO) cDNA was cloned by screening a cDNA library with a human MAO-A cDNA probe. The trout MAO cDNA encodes 499 amino acids, with a molecular mass of 56.6 kDa. The deduced amino acid sequence of trout MAO shows 70% and 71% identity with those of human MAO-A and MAO-B, respectively. Trout MAO contains the pentapeptide sequence Ser-Gly-Gly-Cys-Tyr, to which the cofactor FAD is covalently bound. Transient expression of the cDNA in COS-7 cells shows that trout MAO oxidizes both serotonin [5-hydroxytryptamine (5-HT)] and beta-phenylethylamine (PEA), unlike human MAO-A and MAO-B, which oxidize only 5-HT and PEA, respectively. The Km for 5-HT is similar for trout MAO (130 +/- 17 mM) and human MAO-A (68 +/- 4 mM). The Km for PEA is similar for trout MAO (12.5 +/- 2.0 mM) and human MAO-B (1.5 +/- 0.2 mM). When 5-HT is used as a substrate, trout MAO is more sensitive to clorgyline (IC50, 2.8 +/- 0.2 x 10(-8) M) than deprenyl (IC50, 1.0 +/- 0.1 x 10(-6) M), a result similar to the inhibition selectivity of human MAO-A. However, trout MAO is less sensitive to clorgyline than is human MAO-A (IC50, 5.8 +/- 0.1 x 10(-10) M). Trout MAO is less sensitive to deprenyl (IC50, 4.6 +/- 0.3 x 10(-7) M) than is human MAO-B (IC50, 1.4 +/- 0.1 x 10(-9) M) when PEA is used as the substrate. These results indicate that trout MAO displays substrate and inhibitor selectivities that are not identical to those of either MAO-A and -B, and it therefore represents a novel type of MAO. The structure of trout MAO will provide insights into the substrate and inhibitor selectivities of the MAOs. |
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Authors:
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K Chen; H F Wu; J Grimsby; J C Shih |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular pharmacology Volume: 46 ISSN: 0026-895X ISO Abbreviation: Mol. Pharmacol. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1995-02-02 Completed Date: 1995-02-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0035623 Medline TA: Mol Pharmacol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1226-33 Citation Subset: IM |
Affiliation:
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Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles 90033. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L37878 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA, Complementary Humans Liver / enzymology* Molecular Sequence Data Monoamine Oxidase / genetics* Monoamine Oxidase Inhibitors / pharmacology Sequence Homology, Amino Acid Trout |
| Grant Support | |
ID/Acronym/Agency:
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K05-MH00796/MH/NIMH NIH HHS; R01-MH37020/MH/NIMH NIH HHS; R37-MH39085/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Monoamine Oxidase Inhibitors; EC 1.4.3.4/Monoamine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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