Document Detail

Cloning of the mink plasminogen activator inhibitor type-1 messenger RNA: an mRNA with a short half life.
MedLine Citation:
PMID:  7557448     Owner:  NLM     Status:  MEDLINE    
In mink lung CCL64 epithelial cells the rate of synthesis of plasminogen activator inhibitor type I (PAI-1) increases 10-100-fold within 3 h in response to 12-O-tetradecanoyl phorbol-13-acetate (PMA). The PAI-1 gene is regulated transcriptionally. Parallel studies of the time-courses of PAI-1 synthesis and secretion and of mRNA accumulation indicate that the amount of secreted PAI-1 produced by the cells is tightly coupled to the level of its transcript. The half-life of the PAI-1 mRNA was found to be 25 min which is much shorter than previously reported for PAI-1 in other cells. Actinomycin D, which is commonly used to determine mRNA half-life, stabilized the PAI-1 mRNA. Cycloheximide also stabilized the mRNA. The short half-life and the superinducibility of PAI mRNA are properties shared with rapidly degraded mRNAs encoding protooncoproteins. A 2.97-kb cDNA clone containing the entire coding sequence of PAI-1 was isolated from a cDNA library made from mink lung CCL64 epithelial cells stimulated with PMA. The PAI-1 cDNA contains a long 3'-untranslated region (UTR) of 1720 bp whose sequence is highly conserved among PAI-1 mRNAs from different species. The PAI-1 mRNA also contains several AUUUA pentamer sequences which are the features of an A+U-rich regulatory element such as is found on the fos protooncogene mRNA. Upstream of one of these AUUUA pentamers are several highly conserved sequences that are also found in the 3' UTR of the fos and integrin receptor alpha-subunit mRNAs.(ABSTRACT TRUNCATED AT 250 WORDS)
T H Chuang; R T Hamilton; M Nilsen-Hamilton
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Gene     Volume:  162     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-11-06     Completed Date:  1995-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  303-8     Citation Subset:  IM    
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Line
Cloning, Molecular
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Deoxyadenosines / pharmacology
Enzyme Inhibitors / pharmacology
Gene Expression Regulation, Enzymologic / drug effects
Lung / enzymology
Mink / genetics*
Molecular Sequence Data
Plasminogen Activator Inhibitor 1 / genetics*
Protein Synthesis Inhibitors / pharmacology
RNA, Messenger / genetics,  metabolism
Sequence Alignment
Sequence Homology, Nucleic Acid
Tetradecanoylphorbol Acetate / pharmacology
Transforming Growth Factor beta / pharmacology
Reg. No./Substance:
0/Deoxyadenosines; 0/Enzyme Inhibitors; 0/Plasminogen Activator Inhibitor 1; 0/Protein Synthesis Inhibitors; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 16561-29-8/Tetradecanoylphorbol Acetate; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 73-03-0/cordycepin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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