| Cloning microRNAs from mammalian tissues. | |
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MedLine Citation:
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PMID: 16957376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MicroRNAs (miRNAs) are ubiquitous regulators of gene expression in plants and animals. Their distinctive structure, as very short RNAs with a 5'-phosphate and 3'-hydroxyl group, has enabled the development of protocols to clone miRNAs. After enrichment of these small molecules by size, serial ligation of adapter oligonucleotides to each terminus allows amplification using reverse transcription (RT)-polymerase chain reaction (PCR). Plasmid cloning of multiple miRNA sequences and subsequent DNA sequence analysis enable both bioinformatic characterization of the various miRNAs and experimental validation of their accumulation in cells. |
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Authors:
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Michael Z Michael |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 342 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-09-07 Completed Date: 2006-09-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 189-207 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology and Hepatology, Flinders Medical Center, Flinders University School of Medicine, Bedford Park, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Cloning, Molecular* Computational Biology DNA, Complementary Gene Expression Regulation Humans MicroRNAs / biosynthesis, chemistry, genetics*, isolation & purification Plasmids Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/MicroRNAs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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