Document Detail

Cloning microRNAs from mammalian tissues.
MedLine Citation:
PMID:  16957376     Owner:  NLM     Status:  MEDLINE    
MicroRNAs (miRNAs) are ubiquitous regulators of gene expression in plants and animals. Their distinctive structure, as very short RNAs with a 5'-phosphate and 3'-hydroxyl group, has enabled the development of protocols to clone miRNAs. After enrichment of these small molecules by size, serial ligation of adapter oligonucleotides to each terminus allows amplification using reverse transcription (RT)-polymerase chain reaction (PCR). Plasmid cloning of multiple miRNA sequences and subsequent DNA sequence analysis enable both bioinformatic characterization of the various miRNAs and experimental validation of their accumulation in cells.
Michael Z Michael
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  342     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-07     Completed Date:  2006-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-207     Citation Subset:  IM    
Department of Gastroenterology and Hepatology, Flinders Medical Center, Flinders University School of Medicine, Bedford Park, Australia.
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MeSH Terms
Blotting, Northern
Cloning, Molecular*
Computational Biology
DNA, Complementary
Gene Expression Regulation
MicroRNAs / biosynthesis,  chemistry,  genetics*,  isolation & purification
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA, Complementary; 0/MicroRNAs

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