| Cloning of a melatonin-related receptor from human pituitary. | |
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MedLine Citation:
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PMID: 8647286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have cloned an orphan G protein-coupled receptor from a human pituitary cDNA library using a probe generated by PCR. The cDNA, designated H9, encodes a protein of 613 amino acids that is 45% identical at the amino acid level to the recently cloned human Mel(1a) and Mel(1b) melatonin receptors. Structural analyses of the encoded protein and its gene, along with phylogenetic analysis, further show that H9 is closely related to the G protein-coupled melatonin receptor family. Unusual features of the protein encoded by H9 include a lack of N-linked glycosylation sites and a carboxyl tail >300 amino acids long. H9 transiently expressed in COS-1 cells did not bind [125I]melatonin or [3H]melatonin. H9 mRNA is expressed in hypothalamus and pituitary, suggesting that the encoded receptor and its natural ligand are involved in neuroendocrine function. |
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Authors:
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S M Reppert; D R Weaver; T Ebisawa; C D Mahle; L F Kolakowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 386 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-07-25 Completed Date: 1996-07-25 Revised Date: 2008-05-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 219-24 Citation Subset: IM |
Affiliation:
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Laboratory of Developmental Chronobiology, Massachusetts General Hospital, Boston, MA 02114, USA.reppert@helix.mgh.harvard.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U25341; U52218; U52219; U52220; U52221 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Line, Transformed Cercopithecus aethiops Cloning, Molecular Evolution GTP-Binding Proteins / metabolism Humans Ligands Molecular Sequence Data Nerve Tissue Proteins / chemistry, genetics*, metabolism Pituitary Gland / metabolism* Protein Conformation RNA, Messenger Rats Receptors, Cell Surface / chemistry, genetics*, metabolism Receptors, G-Protein-Coupled* Receptors, Melatonin |
| Grant Support | |
ID/Acronym/Agency:
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R37 HD14427/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/GPR50 protein, human; 0/Ligands; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/Receptors, Melatonin; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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