Document Detail

Cloning of a melatonin-related receptor from human pituitary.
MedLine Citation:
PMID:  8647286     Owner:  NLM     Status:  MEDLINE    
We have cloned an orphan G protein-coupled receptor from a human pituitary cDNA library using a probe generated by PCR. The cDNA, designated H9, encodes a protein of 613 amino acids that is 45% identical at the amino acid level to the recently cloned human Mel(1a) and Mel(1b) melatonin receptors. Structural analyses of the encoded protein and its gene, along with phylogenetic analysis, further show that H9 is closely related to the G protein-coupled melatonin receptor family. Unusual features of the protein encoded by H9 include a lack of N-linked glycosylation sites and a carboxyl tail >300 amino acids long. H9 transiently expressed in COS-1 cells did not bind [125I]melatonin or [3H]melatonin. H9 mRNA is expressed in hypothalamus and pituitary, suggesting that the encoded receptor and its natural ligand are involved in neuroendocrine function.
S M Reppert; D R Weaver; T Ebisawa; C D Mahle; L F Kolakowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  386     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2008-05-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  219-24     Citation Subset:  IM    
Laboratory of Developmental Chronobiology, Massachusetts General Hospital, Boston, MA 02114,
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U25341;  U52218;  U52219;  U52220;  U52221
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Line, Transformed
Cercopithecus aethiops
Cloning, Molecular
GTP-Binding Proteins / metabolism
Molecular Sequence Data
Nerve Tissue Proteins / chemistry,  genetics*,  metabolism
Pituitary Gland / metabolism*
Protein Conformation
RNA, Messenger
Receptors, Cell Surface / chemistry,  genetics*,  metabolism
Receptors, G-Protein-Coupled*
Receptors, Melatonin
Grant Support
Reg. No./Substance:
0/GPR50 protein, human; 0/Ligands; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/Receptors, Melatonin; EC 3.6.1.-/GTP-Binding Proteins

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