| Cloning and identification of porcine programmed death 1. | |
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MedLine Citation:
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PMID: 20347491 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Programmed death 1 (PD-1) is a member of the immunoglobulin (Ig) superfamily, which is expressed on activated T cells, B cells and monocytes. Many researches have demonstrated that a high level of PD-1 expression is closely related to persistent infection and immune evasion in some human infections. In order to study the relationship between PD-1 expression and persistent infections caused by some porcine viruses, we first cloned the porcine PD-1 from porcine PBMCs based on the blast result in the EST database using the human PD-1 sequence. Sequence analysis showed that the cloned PD-1 molecule shares 63 and 54% amino acid sequence identity with human and murine PD-1, respectively. Its molecular structure is also similar to that of human and murine PD-1, containing an IgV-like domain in the extracellular region and two immune regulatory motifs in its cytoplasmic tail. The in vitro T cell proliferation assay showed that the cloned PD-1 could inhibit porcine T cell proliferation by 71% and secretion of IFN-gamma and IL-2 by 64 and 53%, respectively. These data suggest that porcine PD-1 negatively regulates the porcine immune response in a similar manner to that of its counterpart in the human and mouse immune system. |
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Authors:
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Jin-Mei Peng; Zhi-Jun Tian; Heng-Gui Liu; Tong-Qing An; Yan-Jun Zhou; Yao Wang; Deng-Yun Li; Jia-Zeng Chen; Yong-Qian Yang; Guang-Zhi Tong |
Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-07 |
Journal Detail:
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Title: Veterinary immunology and immunopathology Volume: 136 ISSN: 1873-2534 ISO Abbreviation: Vet. Immunol. Immunopathol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 157-62 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antigens, CD / genetics Antigens, Surface / genetics Apoptosis Regulatory Proteins / genetics*, immunology* Base Sequence Cell Proliferation Cloning, Molecular Conserved Sequence DNA Primers / genetics Humans Immunoglobulins / genetics Lymphocyte Activation Mice Molecular Sequence Data Sequence Homology, Amino Acid Sus scrofa / genetics*, immunology* T-Lymphocytes / cytology, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Surface; 0/Apoptosis Regulatory Proteins; 0/DNA Primers; 0/Immunoglobulins; 0/Pdcd1 protein, mouse; 146588-21-8/PDCD1 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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