Document Detail

Cloning of hybridoma cell lines by limiting dilution.
MedLine Citation:
PMID:  18265080     Owner:  NLM     Status:  MEDLINE    
Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.
S A Fuller; M Takahashi; J G Hurrell
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]     Volume:  Chapter 11     ISSN:  1934-3647     ISO Abbreviation:  Curr Protoc Mol Biol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8908160     Medline TA:  Curr Protoc Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit11.8     Citation Subset:  IM    
Allelix Inc., Mississauga, Ontario.
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MeSH Terms
Antibodies, Monoclonal / isolation & purification*
Cloning, Organism / methods*
Hybridomas / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal

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