Document Detail


Cloning of the human dopamine D5 receptor gene and identification of a highly polymorphic microsatellite for the DRD5 locus that shows tight linkage to the chromosome 4p reference marker RAF1P1.
MedLine Citation:
PMID:  8288248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We identified a cosmid clone with exact sequence homology to part of the human dopamine D5 receptor gene (DRD5) after screening a cosmid library with the human DRD1 gene. The dopamine D5 receptor was mapped to chromosome 4p15.1-p15.3 by in situ hybridization and using a somatic cell hybrid panel. We report here the further localization of the DRD5 gene following identification of a highly polymorphic dinucleotide repeat sequence in the cosmid clone. The microsatellite (D5(CT/GT/GA)n) had 12 alleles with a polymorphic information content value of 0.77. Linkage analysis in 39 CEPH pedigrees demonstrated tight linkage to the chromosome 4p reference marker RAF1P1 (Zmaxf 20.66 at theta f 0.05 and ZmaxM 16.57 at theta m 0.07).
Authors:
R Sherrington; B Mankoo; J Attwood; G Kalsi; D Curtis; K Buetow; S Povey; H Gurling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  18     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-02-22     Completed Date:  1994-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  423-5     Citation Subset:  IM    
Affiliation:
Academic Department of Psychiatry, University College London Medical School, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes, Human, Pair 4*
Cloning, Molecular
DNA
DNA, Satellite / genetics*
Female
Genetic Markers
Humans
Linkage (Genetics)
Male
Molecular Sequence Data
Polymorphism, Genetic*
Receptors, Dopamine / genetics*
Receptors, Dopamine D1*
Receptors, Dopamine D5
Chemical
Reg. No./Substance:
0/DNA, Satellite; 0/DRD5 protein, human; 0/Genetic Markers; 0/Receptors, Dopamine; 0/Receptors, Dopamine D1; 137750-35-7/Receptors, Dopamine D5; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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