Document Detail


Cloning and high level expression of gene encoding ES antigen from Trichinella spiralis muscle larvae.
MedLine Citation:
PMID:  7993968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The partial structure gene encoding ES antigen derived from Trichinella spiralis (TSP) muscle larvae was cloned, characterized, and expressed in E. coli. The target DNA (0.7 kb) was directly obtained from the TSP total RNA by using RNA PCR technique. Based on the analysis with the RE digestion, the fragment was cloned into the fusion expression vector pEX31C. It was shown that a kind of 37kDa fusion protein was expressed in E. coli containing the recombinant plasmid by SDS-PAGE electrophoresis. The expressed protein was over 22% of the total cell protein, and it was aggregated in the form of inclusion bodies in E. coli. The purified protein could be recognized in ELISA both by sera from swine-infected with TSP and by the monoclonal antibody against TSP. These findings suggest that the recombinant protein is a potentially valuable antigen both for immunodiagnosis and vaccine development of trichinellosis.
Authors:
Y Yan; W Xu; H Chen; Z Ma; Y Zhu; S Cai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese journal of biotechnology     Volume:  10     ISSN:  1042-749X     ISO Abbreviation:  Chin. J. Biotechnol.     Publication Date:  1994  
Date Detail:
Created Date:  1995-01-18     Completed Date:  1995-01-18     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  9100855     Medline TA:  Chin J Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  IM    
Affiliation:
Institute of Animal and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Helminth / biosynthesis,  genetics*
Blotting, Western
DNA Probes
Escherichia coli / genetics
Helminth Proteins*
Plasmids
Polymerase Chain Reaction
Recombinant Proteins / genetics
Trichinella spiralis / genetics*
Chemical
Reg. No./Substance:
0/Antigens, Helminth; 0/DNA Probes; 0/Helminth Proteins; 0/Recombinant Proteins; 0/TES-26 protein, Toxocara canis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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