Document Detail


Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins.
MedLine Citation:
PMID:  11557513     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The membrane-bound ceruloplasmin homolog hephaestin plays a critical role in intestinal iron absorption. The aims of this study were to clone the rat hephaestin gene and to examine its expression in the gastrointestinal tract in relation to other genes encoding iron transport proteins. The rat hephaestin gene was isolated from intestinal mRNA and was found to encode a protein 96% identical to mouse hephaestin. Analysis by ribonuclease protection assay and Western blotting showed that hephaestin was expressed at high levels throughout the small intestine and colon. Immunofluorescence localized the hephaestin protein to the mature villus enterocytes with little or no expression in the crypts. Variations in iron status had a small but nonsignificant effect on hephaestin expression in the duodenum. The high sequence conservation between rat and mouse hephaestin is consistent with this protein playing a central role in intestinal iron absorption, although its precise function remains to be determined.
Authors:
D M Frazer; C D Vulpe; A T McKie; S J Wilkins; D Trinder; G J Cleghorn; G J Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  281     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-14     Completed Date:  2001-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G931-9     Citation Subset:  IM    
Affiliation:
Joint Clinical Sciences Program, The Queensland Institute of Medical Research and The University of Queensland, PO Royal Brisbane Hospital, Brisbane, Queensland 4029, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Carrier Proteins / genetics,  metabolism
Cation Transport Proteins*
Cloning, Molecular
Digestive System / metabolism*
HLA Antigens / genetics,  metabolism
Histocompatibility Antigens Class I / genetics,  metabolism
Humans
Immunoblotting
Immunohistochemistry
Iron / metabolism*
Iron-Binding Proteins*
Male
Membrane Proteins / chemistry,  genetics,  metabolism*
Mice
Molecular Sequence Data
RNA, Messenger / genetics,  metabolism
Rats
Rats, Sprague-Dawley
Receptors, Transferrin / genetics,  metabolism
Sequence Alignment
Tissue Distribution
Grant Support
ID/Acronym/Agency:
R01 DK-57800-1/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cation Transport Proteins; 0/HEPH protein, human; 0/HFE protein, human; 0/HFE protein, rat; 0/HLA Antigens; 0/Heph protein, mouse; 0/Heph, protein, rat; 0/Hfe protein, mouse; 0/Histocompatibility Antigens Class I; 0/Iron-Binding Proteins; 0/Membrane Proteins; 0/RNA, Messenger; 0/Receptors, Transferrin; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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