Document Detail

Cloning and functional characterization of a fatty acid transport protein (FATP) from the pheromone gland of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone.
MedLine Citation:
PMID:  20875854     Owner:  NLM     Status:  In-Data-Review    
Sex pheromones of moths are largely classified into two types based on the presence (Type I) or absence (Type II) of a terminal functional group. While Type-I sex pheromones are synthesized from common fatty acids in the pheromone gland (PG), Type-II sex pheromones are derived from hydrocarbons produced presumably in the oenocytes and transported to the PG via the hemolymph. Recently, a fatty acid transport protein (BmFATP) was identified from the PG of the silkworm Bombyx mori, which produces a Type-I sex pheromone (bombykol). BmFATP was shown to facilitate the uptake of extracellular fatty acids into PG cells for the synthesis of bombykol. To elucidate the presence and function of FATP in the PG of moths that produce Type-II sex pheromones, we explored fatp homologues expressed in the PG of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone (Type II). A fatp homologue cloned from E. japonica (Ejfatp) was predominantly expressed in the PG, and its expression is upregulated shortly after eclosion. Functional expression of EjFATP in Escherichia coli enhanced the uptake of long chain fatty acids (C(18) and C(20)), but not pheromone precursor hydrocarbons. To the best of our knowledge, this is the first report of the cloning and functional characterization of a FATP in the PG of a moth producing a Type-II sex pheromone. Although EjFATP is not likely to be involved in the uptake of pheromone precursors in E. japonica, the expression pattern of Ejfatp suggests a role for EjFATP in the PG not directly linked to pheromone biosynthesis.
Shuguang Qian; Takeshi Fujii; Katsuhiko Ito; Ryo Nakano; Yukio Ishikawa
Related Documents :
8781494 - Oleic acid-induced mitogenic signaling in vascular smooth muscle cells. a role for prot...
4066594 - Sodium-amino acid cotransport by type ii alveolar epithelial cells.
7582024 - Uptake of amino acids by the parasitic, flagellated protist trichomonas vaginalis.
1692254 - Transport of amino acid amide sarcosinamide and sarcosinamide chloroethylnitrosourea in...
19873974 - Azaindole hydroxamic acids are potent hiv-1 integrase inhibitors.
22853624 - A c-h borylation approach to suzuki-miyaura coupling of typically unstable 2-heteroaryl...
Publication Detail:
Type:  Journal Article     Date:  2010-09-27
Journal Detail:
Title:  Insect biochemistry and molecular biology     Volume:  41     ISSN:  1879-0240     ISO Abbreviation:  Insect Biochem. Mol. Biol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207282     Medline TA:  Insect Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  22-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The potential of liposomes as dental drug delivery systems.
Next Document:  Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armige...