Document Detail


Cloning and functional characterization of Helicobacter pylori fumarate reductase operon comprising three structural genes coding for subunits C, A and B.
MedLine Citation:
PMID:  9434188     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we cloned and sequenced the Helicobacter pylori genes encoding fumarate reductase (FRD). H. pylori frdA, frdB and frdC specify polypeptides of 715, 245 and 254 aa, respectively. The deduced aa sequences of FrdA and FrdB are highly homologous to those of the corresponding subunits of Wolinella succinogenes FRD and also exhibit a significant sequence identity with other bacterial FRD and succinate dehydrogenase subunits A and B. However, H. pylori FrdC shares a striking degree of sequence identity only with W. succinogenes FrdC, which is a cytochrome b with two haem groups. The products encoded by H. pylori frdA, frdB and frdC were overproduced in maxicells and H. pylori FrdA was characterized using an anti-E. coli FrdA serum. H. pylori FRD activity, which was measured as fumarate-dependent benzyl viologen oxidation, is membrane-associated. Inactivation of frdA led to the loss of such activity and the mutant H. pylori cells were delayed (approx. 10-20 h behind their parent cells) in entering the mid-log phase, suggesting that FRD-driven metabolism plays an active but non-essential role for growth of H. pylori cells in vitro. H. pylori FRD contains three subunits, of which FrdA and FrdB appear to form the catalytic dimer, whereas FrdC serves as a membrane anchor.
Authors:
Z Ge; Q Jiang; M S Kalisiak; D E Taylor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  204     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-12     Completed Date:  1998-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-34     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U78101
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antibodies, Bacterial / immunology
Cloning, Molecular
Escherichia coli / enzymology,  immunology
Genes, Bacterial*
Helicobacter pylori / enzymology*
Molecular Sequence Data
Mutagenesis
Operon*
Sequence Homology, Amino Acid
Succinate Dehydrogenase / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; EC 1.3.99.1/Succinate Dehydrogenase

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