| Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase--D-aspartate behavior during germination. | |
| | |
MedLine Citation:
|
PMID: 18318836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The understanding of D-amino acid metabolism in higher plants lags far behind that in mammals, for which the biological functions of these unique amino acids have already been elucidated. In this article, we report on the biochemical behavior of D-amino acids (particularly D-Asp) and relevant metabolic enzymes in Arabidopsis thaliana. During germination and growth of the plant, a transient increase in D-Asp levels was observed, suggesting that D-Asp is synthesized in the plant. Administration of D-Asp suppressed growth, although the inhibitory mechanism responsible for this remains to be clarified. Exogenous D-Asp was efficiently incorporated and metabolized, and was converted to other D-amino acids (D-Glu and D-Ala). We then studied the related metabolic enzymes, and consequently cloned and characterized A. thaliana D-amino acid aminotransferase, which is presumably involved in the metabolism of D-Asp in the plant by catalyzing transamination between D-amino acids. This is the first report of cDNA cloning and functional characterization of a D-amino acid aminotransferase in eukaryotes. The results presented here provide important information for understanding the significance of D-amino acids in the metabolism of higher plants. |
| | |
Authors:
|
Miya Funakoshi; Masae Sekine; Masumi Katane; Takemitsu Furuchi; Masafumi Yohda; Takafumi Yoshikawa; Hiroshi Homma |
Related Documents
:
|
20431016 - D-amino acids trigger biofilm disassembly. 10430856 - Amino acids in the martian meteorite nakhla. 28076 - Extracellular accumulation of a new amino acid, o-2-hydroxypropylhomoserine, from 1,2-p... 3935806 - A multivariate study of the relationship between the genetic code and the physical-chem... 3720946 - Membrane fusion induced by influenza virus hemagglutinin requires protein bound fatty a... 15255756 - Synthesis of chiral 13c,77se-labeled selones. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The FEBS journal Volume: 275 ISSN: 1742-464X ISO Abbreviation: FEBS J. Publication Date: 2008 Mar |
Date Detail:
|
Created Date: 2008-03-05 Completed Date: 2008-05-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101229646 Medline TA: FEBS J Country: England |
Other Details:
|
Languages: eng Pagination: 1188-200 Citation Subset: IM |
Affiliation:
|
School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Arabidopsis / drug effects, enzymology* Arabidopsis Proteins / genetics, metabolism* Bacillus subtilis / enzymology Cloning, Molecular Culture Media / chemistry, metabolism, pharmacology D-Aspartic Acid / analysis, metabolism*, pharmacology Germination* Molecular Sequence Data Recombinant Proteins / genetics, metabolism Transaminases / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Arabidopsis Proteins; 0/Culture Media; 0/Recombinant Proteins; 1783-96-6/D-Aspartic Acid; EC 2.6.1.-/D-amino acid aminotransferase, Arabidopsis thaliana; EC 2.6.1.-/Transaminases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Photosynthetic acclimation: state transitions and adjustment of photosystem stoichiometry--functiona...
Next Document: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human ...