| Cloning and expression of xP1-L, a new marker gene for larval surface mucous cells of tadpole stomach in Xenopus laevis. | |
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MedLine Citation:
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PMID: 17928274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amphibian gastrointestinal tract is remodeled from a larval-type to an adult-type during metamorphosis. In the present study, we examined the products of subtractive hybridization between tadpole and frog stomach cDNAs of Xenopus laevis in order to identify genes expressed specifically in the larval stomach epithelium. A new gene homologous to xP1 was obtained and named xP1-L. In the genome database of Silurana tropicalis, we found a homologue of xP1-L and named it stP1-L. RT-PCR showed that the expression of xP1-L was detected in stage 41/42 tadpoles. In addition, in situ hybridization showed that xP1-L was localized to surface mucous cells of the larval stomach. The H(+)/K(+)-ATPase beta subunit, a marker gene for manicotto gland cells in the tadpole stomach, was also detected at the same time. However, adult marker genes such as xP1 for surface mucous cells and pepsinogen C (PgC) for oxynticopeptic cells were not expressed in the tadpole stages. The expression of xP1-L gradually decreased towards the metamorphic climax and disappeared after stage 61 when larval-type gastric epithelium is replaced by adult-type. We found that xP1-L was never expressed in surface mucous cells of the adult-type stomach, and xP1, instead of xP1-L, was expressed. During T3-induced metamorphosis, xP1-L expression decreased in the same manner as during natural metamorphosis. Thus, xP1-L is a useful marker for larval surface mucous cells in tadpole stomach. This is the first demonstration of a marker gene specific for the surface mucous cells of the larval stomach. |
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Authors:
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Masayuki Ikuzawa; Shigeki Yasumasu; Ken-Ichiro Kobayashi; Ichiro Iuchi |
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Publication Detail:
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Type: Journal Article Date: 2007-09-08 |
Journal Detail:
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Title: Gene expression patterns : GEP Volume: 8 ISSN: 1567-133X ISO Abbreviation: Gene Expr. Patterns Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-16 Completed Date: 2008-02-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101167473 Medline TA: Gene Expr Patterns Country: Netherlands |
Other Details:
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Languages: eng Pagination: 12-8 Citation Subset: IM |
Affiliation:
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Life Science Institute, Sophia University, Tokyo 102-8554, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Cloning, Molecular Gastric Mucosa / chemistry, cytology* Intercellular Signaling Peptides and Proteins / analysis, genetics Larva / cytology* Metamorphosis, Biological Xenopus Proteins / analysis*, genetics Xenopus laevis* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Intercellular Signaling Peptides and Proteins; 0/Xenopus Proteins; 0/xP1 protein, Xenopus; 0/xP1-L protein, Xenopus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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