Document Detail


Cloning and expression of rat fucosyltransferase VII at sites of inflammation.
MedLine Citation:
PMID:  16218937     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sialyl Lewis x (NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc) determinants serve as ligands in the selectin-mediated adhesion of leukocytes to activated endothelium. The final step in the sialyl Lewis x synthesis is catalyzed by alpha1-3-fucosyltransferase, which transfers fucose to sialylated type 2 chain. We report the cloning of rat alpha1-3-fucosyltransferase gene (rFUT) isolated from rat lymph node and kidney allograft. The rFUT is expressed as two splice variants, but only the long one showed enzymatic activity towards sialylated lactosamine. Also flow cytometry analysis with the sLex mAbs indicated that the cloned rFuc-T was a functional enzyme and a member of the Fuc-TVII family. The rFuc-TVII mRNA expression level was strongly enhanced during acute inflammatory reaction induced by kidney allograft rejection, which could be detected by in situ hybridization and quantitative real-time PCR.
Authors:
Jaana Niittymäki; Pirkko Mattila; Risto Renkonen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  113     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-10-12     Completed Date:  2006-01-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  613-20     Citation Subset:  IM    
Affiliation:
Rational Drug Design program, Department of Bacteriology and Immunology, Haartman Institute and Biomedicum, University of Helsinki, Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
COS Cells
Cercopithecus aethiops
Cloning, Molecular
Fucosyltransferases / genetics,  metabolism*
Inflammation / enzymology*
Lymphoid Tissue / enzymology
Molecular Sequence Data
Oligosaccharides / metabolism
RNA, Messenger / metabolism
Rats
Chemical
Reg. No./Substance:
0/5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosamine; 0/Oligosaccharides; 0/RNA, Messenger; EC 2.4.1.-/Fucosyltransferases; EC 2.4.1.152/galactoside 3-fucosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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