Document Detail


Cloning and expression of a lipoxygenase from Pseudomonas aeruginosa 42A2.
MedLine Citation:
PMID:  15803390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to produce (S) 10-monohydroxy-8E-octadecenoic acid (MHOD) from oleic acid, a full-length probable lipoxygenase cDNA from Pseudomonas aeruginosa 42A2 was cloned and expressed in Escherichia coli BL21(DE3). The recombinant protein was purified by affinity chromatography to electrophoretic homogeneity and specifically stained. Its molecular mass was 70 kDa. The activity of the rec-LOX with oleic acid was about 30% of that of the preferred substrate, linoleic acid (100%). Bacterial LOX forms a new subfamily in the lipoxygenase phylogenetic tree.
Authors:
Jaume Vidal-Mas; Montserrat Busquets; Angeles Manresa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  87     ISSN:  0003-6072     ISO Abbreviation:  Antonie Van Leeuwenhoek     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-01     Completed Date:  2005-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  245-51     Citation Subset:  IM    
Affiliation:
Laboratori de Microbiologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona E-08028, Spain.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Affinity
Cloning, Molecular
Conserved Sequence
DNA, Bacterial / chemistry
Escherichia coli / genetics,  metabolism
Linoleic Acid / metabolism
Lipoxygenase / chemistry,  genetics*,  isolation & purification,  metabolism*
Molecular Sequence Data
Molecular Weight
Oleic Acid / metabolism
Phylogeny
Pseudomonas aeruginosa / enzymology*,  genetics*
Recombinant Proteins / chemistry,  genetics,  isolation & purification,  metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Recombinant Proteins; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; EC 1.13.11.12/Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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