Document Detail

Cloning and expression in Escherichia coli of sucrose phosphorylase gene from Leuconostoc mesenteroides No. 165.
MedLine Citation:
PMID:  9063982     Owner:  NLM     Status:  MEDLINE    
The sucrose phosphorylase gene of an isolate, Leuconostoc mesenteroides No. 165, was amplified by PCR, cloned on pUC118, and expressed in E. coli. The nucleotide sequence of the gene showed 96.3% similarity to that of L. mesenteroides ATCC12291 and 67% to that of Streptococcus mutans, but low similarity to the Agrobacterium vitis gene. The cloned gene, which fusing with lacZalpha, was expressed inducibly with IPTG in E. coli to produce an active enzyme in large quantities that accounted for about 50% of the total cell protein.
H Kawasaki; N Nakamura; M Ohmori; T Sakai
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  60     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  322-4     Citation Subset:  B    
Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University, Japan.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / biosynthesis*,  genetics*
Base Sequence
Cloning, Molecular
Escherichia coli / genetics*,  metabolism*
Gene Expression
Glucosyltransferases / biosynthesis*,  genetics*
Leuconostoc / enzymology*,  genetics*
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Reg. No./Substance:
0/Bacterial Proteins; EC 2.4.1.-/Glucosyltransferases; EC phosphorylase

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