| Cloning and expression in Escherichia coli of sucrose phosphorylase gene from Leuconostoc mesenteroides No. 165. | |
| | |
MedLine Citation:
|
PMID: 9063982 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The sucrose phosphorylase gene of an isolate, Leuconostoc mesenteroides No. 165, was amplified by PCR, cloned on pUC118, and expressed in E. coli. The nucleotide sequence of the gene showed 96.3% similarity to that of L. mesenteroides ATCC12291 and 67% to that of Streptococcus mutans, but low similarity to the Agrobacterium vitis gene. The cloned gene, which fusing with lacZalpha, was expressed inducibly with IPTG in E. coli to produce an active enzyme in large quantities that accounted for about 50% of the total cell protein. |
| | |
Authors:
|
H Kawasaki; N Nakamura; M Ohmori; T Sakai |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Bioscience, biotechnology, and biochemistry Volume: 60 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 1996 Feb |
Date Detail:
|
Created Date: 1997-03-14 Completed Date: 1997-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 322-4 Citation Subset: B |
Affiliation:
|
Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Bacterial Proteins / biosynthesis*, genetics* Base Sequence Cloning, Molecular Escherichia coli / genetics*, metabolism* Gene Expression Glucosyltransferases / biosynthesis*, genetics* Leuconostoc / enzymology*, genetics* Molecular Sequence Data Polymerase Chain Reaction Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.7/sucrose phosphorylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Screening for bacteria producing sucrose phosphorylase and characterization of the enzymes.
Next Document: Antimutagenic activity of caffeic acid and related compounds.