Document Detail

Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor.
MedLine Citation:
PMID:  3898078     Owner:  NLM     Status:  MEDLINE    
A murine tumor necrosis factor (MuTNF) cDNA was isolated from a cDNA library prepared by using mRNA from the murine macrophage-like cell line PU5-1.8 induced with 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. The cDNA encodes a polypeptide consisting of a 79 amino acid pre sequence followed by a mature MuTNF sequence of 156 amino acids. The 235 amino acid murine pre-TNF polypeptide is 79% homologous to the human pre-TNF protein. There is one potential N-linked glycosylation site on MuTNF, in contrast to human TNF, which lacks any such site. The MuTNF cDNA, when engineered for expression in Escherichia coli, was found to direct the synthesis of biologically active MuTNF as determined by its cytotoxicity against several transformed cell lines.
D Pennica; J S Hayflick; T S Bringman; M A Palladino; D V Goeddel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  82     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-10-24     Completed Date:  1985-10-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6060-4     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Line
Cloning, Molecular
DNA / genetics
Escherichia coli / genetics
Gene Expression Regulation
Glycoproteins / genetics*
Macrophages / physiology
Molecular Weight
Tumor Necrosis Factor-alpha
Reg. No./Substance:
0/Glycoproteins; 0/Tumor Necrosis Factor-alpha; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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