Document Detail


Cloning and expression of human TRAAK, a polyunsaturated fatty acids-activated and mechano-sensitive K(+) channel.
MedLine Citation:
PMID:  10767409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The two P domain hTRAAK K(+) channel has been cloned from human brain. hTRAAK cDNA encodes a 393 amino acid polypeptide with 88% of homology with its mouse counterpart. The hTRAAK gene has been mapped to chromosome 11q13 and the study of its organization indicates that the hTRAAK open reading frame is contained in six exons. hTRAAK is expressed abundantly in brain and placenta. In COS cells, hTRAAK currents are K(+)-selective, instantaneous and non-inactivating. These currents are insensitive to the classical K(+) channels blockers 4-aminopyridine, tetraethylammonium, barium and quinidine, but are strongly stimulated by application of arachidonic acid as well as other polyunsaturated fatty acids. hTRAAK can also be activated by a stretch of the membrane.
Authors:
F Lesage; F Maingret; M Lazdunski
Related Documents :
12564389 - The role of glycolysis and gluconeogenesis in the cytoprotection of neuroblastoma cells...
2276599 - Increase in the acute toxicity and brain concentrations of chlorophenoxyacetic acids by...
385809 - The role of blood-brain barrier transport of tryptophan and other neutral amino acids i...
18665439 - Acyclovir inhibits rat liver tryptophan-2,3-dioxygenase and induces a concomitant rise ...
41609 - Monoamine mechanisms in chronic schizophrenia: post-mortem neurochemical findings.
47259 - Tissue specificity of nuclear acidic proteins isolated from bovine brain and adrenal me...
7533019 - Effect of alpha-linolenic acid on the metabolism of omega-3 and omega-6 polyunsaturated...
8324099 - Protection by l-ascorbic acid against phototoxicity in tin-protoporphyrin-treated suckl...
8614299 - Functional and ultrastructural effects of essential fatty acid deficiency in kidney epi...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  471     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-18     Completed Date:  2000-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-40     Citation Subset:  IM    
Affiliation:
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS-UPR 411, 660, route des Lucioles, Sophia Antipolis, 06560, Valbonne, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AC005848
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Arachidonic Acid / pharmacology
Base Sequence
Brain / metabolism
COS Cells
Cell Membrane / metabolism
Chromosomes, Human, Pair 11 / genetics
Cloning, Molecular
Electric Conductivity
Exons / genetics
Fatty Acids, Unsaturated / pharmacology*
Humans
Ion Channel Gating / drug effects*
Mice
Molecular Sequence Data
Organ Specificity
Physical Chromosome Mapping
Physical Stimulation
Placenta / metabolism
Potassium / metabolism
Potassium Channels / chemistry,  genetics*,  metabolism*
RNA, Messenger / analysis,  genetics
Substrate Specificity
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/KCNK4 protein, human; 0/Kcnk4 protein, mouse; 0/Potassium Channels; 0/RNA, Messenger; 506-32-1/Arachidonic Acid; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterisation of calmodulin binding to cyclic nucleotide-gated ion channels from Arabidopsis thal...
Next Document:  FHA domain boundaries of the dun1p and rad53p cell cycle checkpoint kinases.