Document Detail

Cloning and expression of human TRAAK, a polyunsaturated fatty acids-activated and mechano-sensitive K(+) channel.
MedLine Citation:
PMID:  10767409     Owner:  NLM     Status:  MEDLINE    
The two P domain hTRAAK K(+) channel has been cloned from human brain. hTRAAK cDNA encodes a 393 amino acid polypeptide with 88% of homology with its mouse counterpart. The hTRAAK gene has been mapped to chromosome 11q13 and the study of its organization indicates that the hTRAAK open reading frame is contained in six exons. hTRAAK is expressed abundantly in brain and placenta. In COS cells, hTRAAK currents are K(+)-selective, instantaneous and non-inactivating. These currents are insensitive to the classical K(+) channels blockers 4-aminopyridine, tetraethylammonium, barium and quinidine, but are strongly stimulated by application of arachidonic acid as well as other polyunsaturated fatty acids. hTRAAK can also be activated by a stretch of the membrane.
F Lesage; F Maingret; M Lazdunski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  471     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-18     Completed Date:  2000-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-40     Citation Subset:  IM    
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS-UPR 411, 660, route des Lucioles, Sophia Antipolis, 06560, Valbonne, France.
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MeSH Terms
Amino Acid Sequence
Arachidonic Acid / pharmacology
Base Sequence
Brain / metabolism
COS Cells
Cell Membrane / metabolism
Chromosomes, Human, Pair 11 / genetics
Cloning, Molecular
Electric Conductivity
Exons / genetics
Fatty Acids, Unsaturated / pharmacology*
Ion Channel Gating / drug effects*
Molecular Sequence Data
Organ Specificity
Physical Chromosome Mapping
Physical Stimulation
Placenta / metabolism
Potassium / metabolism
Potassium Channels / chemistry,  genetics*,  metabolism*
RNA, Messenger / analysis,  genetics
Substrate Specificity
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/KCNK4 protein, human; 0/Kcnk4 protein, mouse; 0/Potassium Channels; 0/RNA, Messenger; 506-32-1/Arachidonic Acid; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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