| Cloning and expression of homospermidine synthase from Senecio vulgaris: a revision. | |
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MedLine Citation:
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PMID: 11117877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Homospermidine synthase. which catalyses the first pathway-specific reaction in pyrrolizidine alkaloid biosynthesis, was cloned from root cultures of Senecio vulgaris and expressed in E. coli. The open reading frame encodes a protein of 370 amino acids with a molecular mass of 40,740 Da. The enzyme is strictly dependent on spermidine as aminobutyl donor since it cannot be substituted by putrescine. The homospermidine synthase from S. vulgaris showed 97.9 and 99.3% nucleic acid identity with two HSS sequences from the closely related species Senecio vernalis. This report also revises data from a previous publication (Kaiser, A., 1999. Cloning and expression of a cDNA encoding homospermidine synthase from Senecio vulgaris (Asteraceae) in Escherichia coli. Plant J. 19. 195 201.) that is incorrect. |
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Authors:
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D Ober; R Harms; T Hartmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytochemistry Volume: 55 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-12-14 Completed Date: 2001-03-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 305-9 Citation Subset: IM |
Affiliation:
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Institut für Pharmazeutische Biologie der Technischen Universität Braunschweig, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkyl and Aryl Transferases
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chemistry,
genetics* Amino Acid Sequence Cloning, Molecular DNA, Complementary Escherichia coli / genetics Molecular Sequence Data Plants, Toxic* Polymerase Chain Reaction Recombinant Proteins / chemistry, genetics Senecio / enzymology* Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Recombinant Proteins; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.1.-/homospermidine synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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