Document Detail


Cloning and expression of homospermidine synthase from Senecio vulgaris: a revision.
MedLine Citation:
PMID:  11117877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Homospermidine synthase. which catalyses the first pathway-specific reaction in pyrrolizidine alkaloid biosynthesis, was cloned from root cultures of Senecio vulgaris and expressed in E. coli. The open reading frame encodes a protein of 370 amino acids with a molecular mass of 40,740 Da. The enzyme is strictly dependent on spermidine as aminobutyl donor since it cannot be substituted by putrescine. The homospermidine synthase from S. vulgaris showed 97.9 and 99.3% nucleic acid identity with two HSS sequences from the closely related species Senecio vernalis. This report also revises data from a previous publication (Kaiser, A., 1999. Cloning and expression of a cDNA encoding homospermidine synthase from Senecio vulgaris (Asteraceae) in Escherichia coli. Plant J. 19. 195 201.) that is incorrect.
Authors:
D Ober; R Harms; T Hartmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  55     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-12-14     Completed Date:  2001-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-9     Citation Subset:  IM    
Affiliation:
Institut für Pharmazeutische Biologie der Technischen Universität Braunschweig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Alkyl and Aryl Transferases / chemistry,  genetics*
Amino Acid Sequence
Cloning, Molecular
DNA, Complementary
Escherichia coli / genetics
Molecular Sequence Data
Plants, Toxic*
Polymerase Chain Reaction
Recombinant Proteins / chemistry,  genetics
Senecio / enzymology*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Recombinant Proteins; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.1.-/homospermidine synthetase

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